Anti-FANCM抗体(ab95014)

概述

  • 产品名称Anti-FANCM抗体
    参阅全部 FANCM 一抗
  • 描述
    兔多克隆抗体to FANCM
  • 经测试应用适用于: ICC/IF, WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Bat, Elephant
  • 免疫原

    A region within N terminal amino acids 1-50 (MSGRQRTLFQ TWGSSISRSS GTPGCSSGTE RPQSPGSSKA PLPAAAEAQL) of Human FANCM (NP_065988.1).

  • 阳性对照
    • HeLa whole cell lysate

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明ab95014 was affinity purified using an epitope specific to FANCM immobilized on solid support.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab95014 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent dilution.
WB 1/2000 - 1/10000. Predicted molecular weight: 227 kDa.
IP Use at 5-15 µg/mg of lysate.

靶标

Anti-FANCM antibody 图像

  • All lanes : Anti-FANCM antibody (ab95014) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg

    Developed using the ECL technique

    Predicted band size : 227 kDa


    Exposure time : 10 seconds
  • ab95014, at 0.4 µg/ml, detecting FANCM in HeLa whole cell lysate by WB following IP.
    Lane 1: ab95014 at 10 µg/mg of lysate
    Lane 2: IP with an antibody which recognizes a downstream epitope of FANCM
    Lane 3: control IgG.
    In each case, 1 mg of lysate was used for IP and 20% of the IP was loaded.
    Detection: Chemiluminescence with an exposure time of 10 seconds
  • ab95014 staining FANCM in HeLa cells by Immunocytochemistry/ Immunofluorescence. Cells were PFA-fixed and permeabilized in 2% Triton X-100 prior to blocking in 7% serum for 1 hour at room temperature. The primary antibody was diluted 1/100 in 0.1% Tween 20 in PBS and incubated with the sample for 17 hours at 4°C. The secondary antibody was Alexa Fluor® 555 conjugated Donkey anti-Rabbit polyclonal, diluted 1/1000.

    See Abreview

Anti-FANCM antibody (ab95014)参考文献

ab95014 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Paraformaldehyde
Permeabilization Yes - 2% TritonX10
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 7% · Temperature: RT°C
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提交于 Mar 09 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"