Synthetic peptide conjugated to KLH derived from within residues 850 - 950 of Human FANCM.
(Peptide available as ab27788.)
- This antibody gave a positive signal in the following whole cell lysates; HeLa, MDA-MB-361 and WI38.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use at 1 µg/mg of lysate. (see Abreview)
||Use a concentration of 1 mg/ml. Detects a band of approximately 240 kDa (predicted molecular weight: 232 kDa).
FANCM is an ATPase required for FANCD2 ubiquitination, a key reaction in DNA repair. It binds to ssDNA but not to dsDNA.
- ATP-dependent RNA helicase FANCM antibody
- FAAP250 antibody
- Fanconi anemia group M protein antibody
Western blot - Anti-FANCM antibody (ab26272)
All lanes :
Anti-FANCM antibody (ab26272) at 1 µg/mlLane 1 :
HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 2 :
MDA-MB-361 (Human breast adenocarcinoma cell line) Whole Cell LysateLane 3 :
WI38 (Human lung fibroblast cell line) Whole Cell Lysate
Lysates/proteins at 20 µg per lane.SecondaryAll lanes :
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.Predicted band size:
232 kDaObserved band size:
238 kDa (why is the actual band size different from the predicted?
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