The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 43 kDa).
功能Ubiquitin ligase protein that mediates monoubiquitination of FANCD2, a key step in the DNA damage pathway. Also mediates monoubiquitination of FANCI. May stimulate the ubiquitin release from UBE2W. May be required for proper primordial germ cell proliferation in the embryonic stage, whereas it is probably not needed for spermatogonial proliferation after birth.
通路Protein modification; protein ubiquitination.
疾病相关Fanconi anemia complementation group L
序列相似性Contains 1 RING-type zinc finger.
结构域The UBC-RWD region (URD) region mediates interaction with FANCI and FANCD2.
翻译后修饰The RING-type zinc finger domain is monoubiquitinated in the presence of UBE2T and UBE2W.