概述

  • 产品名称
  • 描述
    兔多克隆抗体to FANCI
  • 经测试应用
    适用于: WB, IPmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Horse, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Bat
  • 免疫原

    Synthetic peptide corresponding to a region between residue 1025 and 1075 of human KIAA1794 (NP_060663.2)

  • 阳性对照
    • Whole cell lysates from HeLa and 293T cells.

性能

应用

Our Abpromise guarantee covers the use of ab74332 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Predicted molecular weight: 149 kDa.
IP Use at 2-5 µg/mg of lysate.

靶标

  • 功能
    Required for maintenance of chromosomal stability. Involved in the repair of DNA double-strand breaks by homologous recombination and in the repair of DNA cross-links. Participates in S phase and G2 phase checkpoint activation upon DNA damage. Promotes FANCD2 ubiquitination and recruitment to DNA repair sites.
  • 疾病相关
    Defects in FANCI are a cause of Fanconi anemia complementation group I (FANCI) [MIM:609053]. It is a disorder affecting all bone marrow elements and resulting in anemia, leukopenia and thrombopenia. It is associated with cardiac, renal and limb malformations, dermal pigmentary changes, and a predisposition to the development of malignancies. At the cellular level it is associated with hypersensitivity to DNA-damaging agents, chromosomal instability (increased chromosome breakage) and defective DNA repair.
  • 结构域
    The C-terminal 30 residues are probably required for function in DNA repair.
  • 翻译后修饰
    Monoubiquitinated on Lys-523 during S phase and upon genotoxic stress. Deubiquitinated by USP1 as cells enter G2/M, or once DNA repair is completed. Monoubiquitination requires the FANCA-FANCB-FANCC-FANCE-FANCF-FANCG-FANCM complex. Ubiquitination is required for binding to chromatin, DNA repair, and normal cell cycle progression.
    Phosphorylated in response to DNA damage by ATM and/or ATR.
  • 细胞定位
    Nucleus. Concentrates in nuclear foci upon genotoxic stress.
  • Information by UniProt
  • 数据库链接
  • 别名
    • FANCI antibody
    • FANCI gene antibody
    • FANCI_HUMAN antibody
    • Fanconi anemia group I protein antibody
    • Fanconi anemia, complementation group I antibody
    • FLJ10719 antibody
    • FLJ14658 antibody
    • KIAA1794 antibody
    • Protein FACI antibody
    • Protein FANCI antibody
    see all

Anti-FANCI antibody 图像

  • All lanes : Anti-FANCI antibody (ab74332) at 0.04 µg/ml

    Lane 1 : Whole HeLa cell lysate at 50 µg
    Lane 2 : Whole HeLa cell lysate at 15 µg
    Lane 3 : Whole HeLa cell lysate at 5 µg
    Lane 4 : Whole 293T cell lysate at 50 µg


    Predicted band size : 149 kDa
    Additional bands at : 100 kDa,65 kDa. We are unsure as to the identity of these extra bands.
  • Detection of Human KIAA1794 by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab74332 at 3 µg/mg for IP (Lane 1) and at 1 µg/ml for subsequent WB detection. Lane 2 represents IgG control IP.

Anti-FANCI antibody (ab74332)参考文献

This product has been referenced in:
  • Jaber S  et al. p53 downregulates the Fanconi anaemia DNA repair pathway. Nat Commun 7:11091 (2016). WB . Read more (PubMed: 27033104) »
  • Luebben SW  et al. A concomitant loss of dormant origins and FANCC exacerbates genome instability by impairing DNA replication fork progression. Nucleic Acids Res 42:5605-15 (2014). ICC/IF ; Mouse . Read more (PubMed: 24589582) »

See all 4 Publications for this product

Product Wall

There are currently no Abreviews or Questions for ab74332.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册