概述

  • 产品名称
  • 描述
    兔多克隆抗体to FANCI
  • 经测试应用
    适用于: ICC/IF, IP, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide within Human FANCI aa 200-250. The exact sequence is proprietary.
    Database link: Q9NVI1

  • 阳性对照
    • WB: HeLa cell nuclear extract. ICC/IF: HeLa cells.

性能

应用

Our Abpromise guarantee covers the use of ab15344 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration. PubMed: 19465922
IP Use at 1-4 µg/mg of lysate.
WB 1/2500 - 1/10000. Detects a band of approximately 145 kDa (predicted molecular weight: 149, 142, 27 kDa).

靶标

  • 功能
    Required for maintenance of chromosomal stability. Involved in the repair of DNA double-strand breaks by homologous recombination and in the repair of DNA cross-links. Participates in S phase and G2 phase checkpoint activation upon DNA damage. Promotes FANCD2 ubiquitination and recruitment to DNA repair sites.
  • 疾病相关
    Defects in FANCI are a cause of Fanconi anemia complementation group I (FANCI) [MIM:609053]. It is a disorder affecting all bone marrow elements and resulting in anemia, leukopenia and thrombopenia. It is associated with cardiac, renal and limb malformations, dermal pigmentary changes, and a predisposition to the development of malignancies. At the cellular level it is associated with hypersensitivity to DNA-damaging agents, chromosomal instability (increased chromosome breakage) and defective DNA repair.
  • 结构域
    The C-terminal 30 residues are probably required for function in DNA repair.
  • 翻译后修饰
    Monoubiquitinated on Lys-523 during S phase and upon genotoxic stress. Deubiquitinated by USP1 as cells enter G2/M, or once DNA repair is completed. Monoubiquitination requires the FANCA-FANCB-FANCC-FANCE-FANCF-FANCG-FANCM complex. Ubiquitination is required for binding to chromatin, DNA repair, and normal cell cycle progression.
    Phosphorylated in response to DNA damage by ATM and/or ATR.
  • 细胞定位
    Nucleus. Concentrates in nuclear foci upon genotoxic stress.
  • Information by UniProt
  • 数据库链接
  • 别名
    • FANCI antibody
    • FANCI gene antibody
    • FANCI_HUMAN antibody
    • Fanconi anemia group I protein antibody
    • Fanconi anemia, complementation group I antibody
    • FLJ10719 antibody
    • FLJ14658 antibody
    • KIAA1794 antibody
    • Protein FACI antibody
    • Protein FANCI antibody
    see all

图片

  • ab15344 (1/400) staining FANCI in HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilized with 0.5% Triton X-100/PBS and counterstained with DAPI in order to highlight the nucleus (red). for further experimental details please refer to Abreview.

    See Abreview

  • All lanes : Anti-FANCI antibody (ab15344) at 1/5000 dilution

    Lane 1 : HeLa nuclear extract at 5 µg
    Lane 2 : HeLa nuclear extract at 20 µg

    Developed using the ECL technique

    Predicted band size : 149, 142, 27 kDa


    Exposure time : 15 minutes

文献

This product has been referenced in:
  • Tan SLW  et al. A Class of Environmental and Endogenous Toxins Induces BRCA2 Haploinsufficiency and Genome Instability. Cell 169:1105-1118.e15 (2017). Read more (PubMed: 28575672) »
  • Pourfarzad F  et al. Locus-specific proteomics by TChP: targeted chromatin purification. Cell Rep 4:589-600 (2013). WB ; Human . Read more (PubMed: 23911284) »

See all 7 Publications for this product

客户评价及客户问答

Thank you for submitting an Abreview of ab15344. As you may have noticed, your review has now been published on our website.

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Thank you for your interest in our antibodies. We do not provide samples of our antibodies as under our Abpromise we guarantee our antibodies to work as stated on the data sheet. In the case of ab15344, ab74332 and ab15346as they are only guaranteed to...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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