This fast track antibody is not yet fully characterized. It is subject to these terms and conditions

Anti-FANCG (phospho S383)抗体(ab31893)

概述

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

  • 应用说明This antibody gave a positive result in ELISA against the immunizing peptide (ab33127). It gave a negative result in ELISA against the non-modified equivalent peptide (ab42660). This indicates that it is specific for the modified peptide.
    See figure below.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能DNA repair protein that may operate in a postreplication repair or a cell cycle checkpoint function. May be implicated in interstrand DNA cross-link repair and in the maintenance of normal chromosome stability. Candidate tumor suppressor gene.
    • 组织特异性Highly expressed in testis and thymus. Found in lymphoblasts.
    • 疾病相关Defects in FANCG are a cause of Fanconi anemia (FA) [MIM:227650]. FA is a genetically heterogeneous, autosomal recessive disorder characterized by progressive pancytopenia, a diverse assortment of congenital malformations, and a predisposition to the development of malignancies. At the cellular level it is associated with hypersensitivity to DNA-damaging agents, chromosomal instability (increased chromosome breakage), and defective DNA repair.
    • 序列相似性Contains 4 TPR repeats.
    • 细胞定位Nucleus. Cytoplasm. The major form is nuclear. The minor form is cytoplasmic.
    • Information by UniProt
    • 数据库链接
    • 别名
      • DNA repair protein XRCC9 antibody
      • FAG antibody
      • FANCG antibody
      • FANCG_HUMAN antibody
      • Fanconi anaemia complementation group G antibody
      • Fanconi anemia group G protein antibody
      • Protein FACG antibody
      • X ray repair, complementing defective, in Chinese hamster cells 9 antibody
      • X-ray repair, complementing defective, in Chinese hamster, 9 antibody
      • XRCC9 antibody
      see all

    Anti-FANCG (phospho S383) antibody 图像

    This Fast-Track antibody is not yet fully characterised. These images represent inconclusive preliminary data.

    • This antibody gave a positive result in ELISA against the immunizing peptide (ab33127, blue). It gave a negative result in ELISA against the non-modified equivalent peptide (ab42660, purple). This indicates that it is specific for the modified peptide.
          

    • CC/IF image of ab31893 stained human HepG2 cells. The cells were 4% PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab31893, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in HeLa, HEK 293 and MCF7 cells.  However, this Fast-Track antibody is not yet fully characterised.  This image represents inconclusive preliminary data.

    Anti-FANCG (phospho S383) antibody (ab31893)参考文献

    ab31893 has not yet been referenced specifically in any publications.

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