Anti-FANCE抗体(ab83851)

概述

  • 产品名称
  • 描述
    兔多克隆抗体to FANCE
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Horse, Cow
  • 免疫原

    Synthetic peptide corresponding to a region within internal sequence amino acids 180-229 (SPQAPDPEEE ENRDSQQPGK RRKDSEEEAA SPEGKRVPKR LRCWEEEEDH) of human FANCE (NP_068741).

  • 阳性对照
    • HepG2 cell lysate.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    ab83851 is purified by a peptide affinity chromatography method.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab83851 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 59 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能
    As part of the Fanconi anemia (FA) complex functions in DNA cross-links repair. Required for the nuclear accumulation of FANCC and provides a critical bridge between the FA complex and FANCD2.
  • 疾病相关
    Fanconi anemia complementation group E
  • 翻译后修饰
    Phosphorylated. Phosphorylation by CHEK1 at Thr-346 and Ser-374 regulates its function in DNA cross-links repair.
    Ubiquitinated. Phosphorylation by CHEK1 induces polyubiquitination and degradation.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 2810451D06Rik antibody
    • AI415634 antibody
    • AW209126 antibody
    • FACE antibody
    • FAE antibody
    • FANCE antibody
    • FANCE_HUMAN antibody
    • Fanconi anemia complementation group E antibody
    • Fanconi anemia group E protein antibody
    • OTTHUMP00000016254 antibody
    • Protein FACE antibody
    • RGD1561045 antibody
    see all

Anti-FANCE antibody 图像

  • Anti-FANCE antibody (ab83851) at 1 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 59 kDa
    Observed band size : 59 kDa

实验方案

Anti-FANCE antibody (ab83851)参考文献

ab83851 has not yet been referenced specifically in any publications.

Product Wall

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Paraformaldehyde
Permeabilization
Yes - 2% TritonX100
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 7% · Temperature: RT°C
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提交于 Mar 23 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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