Anti-FANCD2 (phospho S222)抗体(ab53761)

概述

  • 产品名称Anti-FANCD2 (phospho S222)抗体
    参阅全部 FANCD2 一抗
  • 描述
    兔多克隆抗体to FANCD2 (phospho S222)
  • 特异性ab53761 detects endogenous levels of FANCD2 only when phosphorylated at serine 222.
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic phosphopeptide derived from human FANCD2 around the phosphorylation site of serine 222 (G-D-SP-Q-H).

  • 阳性对照
    • WB: HT29 cell extracts, treated with Calyculin A (50ng/ml, 30mins).

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明ab53761 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab53761 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 166 kDa (predicted molecular weight: 166 kDa).
ELISA 1/5000.

靶标

  • 功能Required for maintenance of chromosomal stability. Promotes accurate and efficient pairing of homologs during meiosis. Involved in the repair of DNA double-strand breaks, both by homologous recombination and single-strand annealing. May participate in S phase and G2 phase checkpoint activation upon DNA damage. Promotes BRCA2/FANCD1 loading onto damaged chromatin. May also be involved in B-cell immunoglobulin isotype switching.
  • 组织特异性Highly expressed in germinal center cells of the spleen, tonsil, and reactive lymph nodes, and in the proliferating basal layer of squamous epithelium of tonsil, esophagus, oropharynx, larynx and cervix. Expressed in cytotrophoblastic cells of the placenta and exocrine cells of the pancreas (at protein level). Highly expressed in testis, where expression is restricted to maturing spermatocytes.
  • 疾病相关Defects in FANCD2 are a cause of Fanconi anemia complementation group D type 2 (FANCD2) [MIM:227646]. It is a disorder affecting all bone marrow elements and resulting in anemia, leukopenia and thrombopenia. It is associated with cardiac, renal and limb malformations, dermal pigmentary changes, and a predisposition to the development of malignancies. At the cellular level it is associated with hypersensitivity to DNA-damaging agents, chromosomal instability (increased chromosome breakage) and defective DNA repair.
  • 发展阶段Highly expressed in fetal oocytes, and in hematopoietic cells of the fetal liver and bone marrow (at protein level).
  • 结构域The C-terminal 24 residues of isoform 2 are required for its function.
  • 翻译后修饰Monoubiquitinated on Lys-561 during S phase and upon genotoxic stress (isoform 1 and isoform 2). Deubiquitinated by USP1 as cells enter G2/M, or once DNA repair is completed. Monoubiquitination requires the FANCA-FANCB-FANCC-FANCE-FANCF-FANCG-FANCM complex, RPA1 and ATR, and is mediated by FANCL/PHF9. Ubiquitination is required for binding to chromatin, interaction with BRCA1, BRCA2 and MTMR15/FAN1, DNA repair, and normal cell cycle progression, but not for phosphorylation on Ser-222 or interaction with MEN1.
    Phosphorylated in response to various genotoxic stresses by ATM and/or ATR. Upon ionizing radiation, phosphorylated by ATM on Ser-222 and Ser-1404. Phosphorylation on Ser-222 is required for S-phase checkpoint activation, but not for ubiquitination, foci formation, or DNA repair. In contrast, phosphorylation by ATR on other sites may be required for ubiquitination and foci formation.
  • 细胞定位Nucleus. Concentrates in nuclear foci during S phase and upon genotoxic stress.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DKFZp762A223 antibody
    • FA 4 antibody
    • FA D2 antibody
    • FA4 antibody
    • FAC D2 antibody
    • FACD 2 antibody
    • FACD antibody
    • FACD2 antibody
    • FACD2_HUMAN antibody
    • FAD antibody
    • FAD2 antibody
    • FANC D2 antibody
    • FANCD 2 antibody
    • FANCD antibody
    • FANCD2 antibody
    • FANCONI ANEMIA COMPLEMENTATION GROUP D antibody
    • Fanconi anemia complementation group D2 antibody
    • Fanconi anemia group D2 protein antibody
    • FANCONI PANCYTOPENIA TYPE 4 antibody
    • FLJ23826 antibody
    • OTTHUMP00000158853 antibody
    • OTTHUMP00000207925 antibody
    • Protein FACD2 antibody
    • Type 4 Fanconi pancytopenia antibody
    see all

Anti-FANCD2 (phospho S222) antibody 图像

  • All lanes : Anti-FANCD2 (phospho S222) antibody (ab53761) at 1/500 dilution

    Lane 1 : HT29 cell extract, treated with Calyculin A (50ng/ml, 30mins)
    Lane 2 : HT29 cell extract, treated with Calyculin A (50ng/ml, 30mins) with immunising peptide


    Predicted band size : 166 kDa
    Observed band size : 166 kDa

Anti-FANCD2 (phospho S222) antibody (ab53761)参考文献

ab53761 has not yet been referenced specifically in any publications.

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