概述

  • 产品名称Anti-FANCD2抗体[EPR2302]
    参阅全部 FANCD2 一抗
  • 描述
    兔单克隆抗体[EPR2302] to FANCD2
  • 经测试应用适用于: IHC-P, ICC/IF, IP, WBmore details
    不适用于: Flow Cyt
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to residues in Human FANCD2.

  • 阳性对照
    • HeLa, MCF7, SKBR-3, K562, HL-60, C6, and PC-12 cell lysates, Human tonsil tissue, HeLa cells ICC/IF: HAP1 cells and FANCD2-HAP1 knockout cells
  • 常规说明

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

    This product is a recombinant rabbit monoclonal antibody.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
  • 存储溶液PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • 纯度Tissue culture supernatant
  • 克隆单克隆
  • 克隆编号EPR2302
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab108928 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/250.
IP 1/10 - 1/100.
WB 1/1000 - 1/10000. Detects a band of approximately 155 kDa (predicted molecular weight: 166 kDa).
  • 应用说明Is unsuitable for Flow Cyt.
  • 靶标

    • 功能Required for maintenance of chromosomal stability. Promotes accurate and efficient pairing of homologs during meiosis. Involved in the repair of DNA double-strand breaks, both by homologous recombination and single-strand annealing. May participate in S phase and G2 phase checkpoint activation upon DNA damage. Promotes BRCA2/FANCD1 loading onto damaged chromatin. May also be involved in B-cell immunoglobulin isotype switching.
    • 组织特异性Highly expressed in germinal center cells of the spleen, tonsil, and reactive lymph nodes, and in the proliferating basal layer of squamous epithelium of tonsil, esophagus, oropharynx, larynx and cervix. Expressed in cytotrophoblastic cells of the placenta and exocrine cells of the pancreas (at protein level). Highly expressed in testis, where expression is restricted to maturing spermatocytes.
    • 疾病相关Defects in FANCD2 are a cause of Fanconi anemia complementation group D type 2 (FANCD2) [MIM:227646]. It is a disorder affecting all bone marrow elements and resulting in anemia, leukopenia and thrombopenia. It is associated with cardiac, renal and limb malformations, dermal pigmentary changes, and a predisposition to the development of malignancies. At the cellular level it is associated with hypersensitivity to DNA-damaging agents, chromosomal instability (increased chromosome breakage) and defective DNA repair.
    • 发展阶段Highly expressed in fetal oocytes, and in hematopoietic cells of the fetal liver and bone marrow (at protein level).
    • 结构域The C-terminal 24 residues of isoform 2 are required for its function.
    • 翻译后修饰Monoubiquitinated on Lys-561 during S phase and upon genotoxic stress (isoform 1 and isoform 2). Deubiquitinated by USP1 as cells enter G2/M, or once DNA repair is completed. Monoubiquitination requires the FANCA-FANCB-FANCC-FANCE-FANCF-FANCG-FANCM complex, RPA1 and ATR, and is mediated by FANCL/PHF9. Ubiquitination is required for binding to chromatin, interaction with BRCA1, BRCA2 and MTMR15/FAN1, DNA repair, and normal cell cycle progression, but not for phosphorylation on Ser-222 or interaction with MEN1.
      Phosphorylated in response to various genotoxic stresses by ATM and/or ATR. Upon ionizing radiation, phosphorylated by ATM on Ser-222 and Ser-1404. Phosphorylation on Ser-222 is required for S-phase checkpoint activation, but not for ubiquitination, foci formation, or DNA repair. In contrast, phosphorylation by ATR on other sites may be required for ubiquitination and foci formation.
    • 细胞定位Nucleus. Concentrates in nuclear foci during S phase and upon genotoxic stress.
    • Information by UniProt
    • 数据库链接
    • 别名
      • DKFZp762A223 antibody
      • FA 4 antibody
      • FA D2 antibody
      • FA4 antibody
      • FAC D2 antibody
      • FACD 2 antibody
      • FACD antibody
      • FACD2 antibody
      • FACD2_HUMAN antibody
      • FAD antibody
      • FAD2 antibody
      • FANC D2 antibody
      • FANCD 2 antibody
      • FANCD antibody
      • FANCD2 antibody
      • FANCONI ANEMIA COMPLEMENTATION GROUP D antibody
      • Fanconi anemia complementation group D2 antibody
      • Fanconi anemia group D2 protein antibody
      • FANCONI PANCYTOPENIA TYPE 4 antibody
      • FLJ23826 antibody
      • OTTHUMP00000158853 antibody
      • OTTHUMP00000207925 antibody
      • Protein FACD2 antibody
      • Type 4 Fanconi pancytopenia antibody
      see all

    Anti-FANCD2 antibody [EPR2302] 图像



    • Predicted band size : 166 kDa

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: FANCD2 knockout HAP1 cell lysate (20 µg)
      Lane 3: HeLa cell lysate (20 µg)
      Lane 4: HEK293 cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab108928 observed at 165 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab108928 was shown to specifically react with FANCD2 when FANCD2 knockout samples were used. Wild-type and FANCD2 knockout samples were subjected to SDS-PAGE. ab108928 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) ab216776 secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

    • ab108928 staining FANCD2 in wild-type HAP1 cells (top panel) and FANCD2 knockout HAP1 cells (bottom panel). The cells were fixed with 4% formaldehyde (10min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab108928 at 1/250 dilution and ab195889 at 1/250 dilution (shown in pseudo colour red) overnight at +4°C, followed by a further incubation at room temperature for 1h with a goat secondary antibody to Rabbit IgG (Alexa Fluor® 488) (ab150081) at 2 μg/ml (shown in green). Nuclear DNA was labelled in blue with DAPI.

    • ab108928 staining FANCD2 in Human U2OS osteosarcoma cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.5% Triton X-100 and blocked with 10% goat serum for 1 hour at 20°C. Samples were incubated with primary antibody (1/300) for 18 hours at 4°C. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG monoclonal (1/1000) was used as the secondary antibody.

      See Abreview

    • All lanes : Anti-FANCD2 antibody [EPR2302] (ab108928) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : MCF7 cell lysate
      Lane 3 : SKBR-3 cell lysate
      Lane 4 : K562 cell lysate
      Lane 5 : HL60 cell lysate
      Lane 6 : C6 cell lysate
      Lane 7 : PC-12 cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 166 kDa
    • Immunohistochemical staining of paraffin-embedded Human tonsil tissue using ab108928 at a dilution of 1/100.
    • Immunofluorescent staining of HeLa cells using ab108928 at a dilution of 1/250.

    Anti-FANCD2 antibody [EPR2302] (ab108928)参考文献

    This product has been referenced in:
    • Jiang Q  et al. MERIT40 cooperates with BRCA2 to resolve DNA interstrand cross-links. Genes Dev 29:1955-68 (2015). Human . Read more (PubMed: 26338419) »
    • Xu W  et al. TXNL1-XRCC1 pathway regulates cisplatin-induced cell death and contributes to resistance in human gastric cancer. Cell Death Dis 5:e1055 (2014). WB ; Human . Read more (PubMed: 24525731) »

    See all 7 Publications for this product

    Product Wall

    Application Immunocytochemistry/ Immunofluorescence
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
    Sample Human Cell (u2os osteosarcoma)
    Specification u2os osteosarcoma
    Permeabilization Yes - 0.5% triton
    Fixative Paraformaldehyde
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    提交于 Apr 25 2014

    Application Western blot
    Loading amount 30 µg
    Gel Running Conditions Reduced Denaturing (6%)
    Sample Mouse Cell lysate - whole cell (Mouse Pancreatic cancer)
    Specification Mouse Pancreatic cancer
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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    提交于 Mar 18 2014

    Application Western blot
    Loading amount 20 µg
    Gel Running Conditions Reduced Denaturing (6%)
    Sample Human Tissue lysate - whole (U2OS osteosarcoma)
    Specification U2OS osteosarcoma
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Username

    Abcam user community

    Verified customer

    提交于 Mar 04 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"