Anti-FANCD2抗体(Biotin) (ab81768)

概述

  • 产品名称Anti-FANCD2抗体(Biotin)
    参阅全部 FANCD2 一抗
  • 描述
    兔多克隆抗体to FANCD2 (Biotin)
  • 偶联物Biotin
  • 特异性ab81768 is specific for FANCD2.
  • 经测试应用适用于: WB, IP, ICC/IF, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Human FANCD2 fusion protein (N terminal fragment)

  • 阳性对照
    • HeLa cells extract. Human MMC and IR treated MEF cells. Human prostate, glandular epithelium. SiHa and U2OS cells exposed to IR.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: None
    Constituents: 1% BSA, PBS
  • Concentration information loading...
  • 纯度Protein A purified
  • 纯化说明Affinity purified rabbit antisera.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab81768 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/10000 - 1/20000. Predicted molecular weight: 166 kDa.
IP Use at an assay dependent dilution.
ICC/IF 1/200 - 1/500.
IHC-P Use at an assay dependent dilution.

靶标

  • 功能Required for maintenance of chromosomal stability. Promotes accurate and efficient pairing of homologs during meiosis. Involved in the repair of DNA double-strand breaks, both by homologous recombination and single-strand annealing. May participate in S phase and G2 phase checkpoint activation upon DNA damage. Promotes BRCA2/FANCD1 loading onto damaged chromatin. May also be involved in B-cell immunoglobulin isotype switching.
  • 组织特异性Highly expressed in germinal center cells of the spleen, tonsil, and reactive lymph nodes, and in the proliferating basal layer of squamous epithelium of tonsil, esophagus, oropharynx, larynx and cervix. Expressed in cytotrophoblastic cells of the placenta and exocrine cells of the pancreas (at protein level). Highly expressed in testis, where expression is restricted to maturing spermatocytes.
  • 疾病相关Defects in FANCD2 are a cause of Fanconi anemia complementation group D type 2 (FANCD2) [MIM:227646]. It is a disorder affecting all bone marrow elements and resulting in anemia, leukopenia and thrombopenia. It is associated with cardiac, renal and limb malformations, dermal pigmentary changes, and a predisposition to the development of malignancies. At the cellular level it is associated with hypersensitivity to DNA-damaging agents, chromosomal instability (increased chromosome breakage) and defective DNA repair.
  • 发展阶段Highly expressed in fetal oocytes, and in hematopoietic cells of the fetal liver and bone marrow (at protein level).
  • 结构域The C-terminal 24 residues of isoform 2 are required for its function.
  • 翻译后修饰Monoubiquitinated on Lys-561 during S phase and upon genotoxic stress (isoform 1 and isoform 2). Deubiquitinated by USP1 as cells enter G2/M, or once DNA repair is completed. Monoubiquitination requires the FANCA-FANCB-FANCC-FANCE-FANCF-FANCG-FANCM complex, RPA1 and ATR, and is mediated by FANCL/PHF9. Ubiquitination is required for binding to chromatin, interaction with BRCA1, BRCA2 and MTMR15/FAN1, DNA repair, and normal cell cycle progression, but not for phosphorylation on Ser-222 or interaction with MEN1.
    Phosphorylated in response to various genotoxic stresses by ATM and/or ATR. Upon ionizing radiation, phosphorylated by ATM on Ser-222 and Ser-1404. Phosphorylation on Ser-222 is required for S-phase checkpoint activation, but not for ubiquitination, foci formation, or DNA repair. In contrast, phosphorylation by ATR on other sites may be required for ubiquitination and foci formation.
  • 细胞定位Nucleus. Concentrates in nuclear foci during S phase and upon genotoxic stress.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DKFZp762A223 antibody
    • FA 4 antibody
    • FA D2 antibody
    • FA4 antibody
    • FAC D2 antibody
    • FACD 2 antibody
    • FACD antibody
    • FACD2 antibody
    • FACD2_HUMAN antibody
    • FAD antibody
    • FAD2 antibody
    • FANC D2 antibody
    • FANCD 2 antibody
    • FANCD antibody
    • FANCD2 antibody
    • FANCONI ANEMIA COMPLEMENTATION GROUP D antibody
    • Fanconi anemia complementation group D2 antibody
    • Fanconi anemia group D2 protein antibody
    • FANCONI PANCYTOPENIA TYPE 4 antibody
    • FLJ23826 antibody
    • OTTHUMP00000158853 antibody
    • OTTHUMP00000207925 antibody
    • Protein FACD2 antibody
    • Type 4 Fanconi pancytopenia antibody
    see all

Anti-FANCD2 antibody (Biotin) 图像

  • Immunofluorescence analysis using a 1/200 dilution of ab81768. FANCD2 colocalizes in vivo with another protein in SiHa cells after cell exposure to IR. Proliferating SiHa cells were exposed to 10 Gy of IR and double color immunofluorescence staining was performed after 8 h. Images were captured in a Kodak digital image system on a Leica fluorescence microscope.
  • Immunfluorescence analysis using a 1/200 dilution of ab81768. FANCD2 colocalizes in vivo with another protein in U2OS cells after cell exposure to IR. Proliferating U2OS cells were exposed to 10 Gy of IR and double color immunofluorescence staining was performed after 8 h. Images were captured in a Kodak digital image system on a Leica fluorescence microscope.
  • Anti-FANCD2 antibody (Biotin) (ab81768) at 1/10000 dilution + HeLa whole cell extract

    Predicted band size : 166 kDa
    Observed band size : 166 kDa
    Additional bands at : 150 kDa,<50 kDa. We are unsure as to the identity of these extra bands.

Anti-FANCD2 antibody (Biotin) (ab81768)参考文献

ab81768 has not yet been referenced specifically in any publications.

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