概述

  • 产品名称Anti-FANCC抗体
    参阅全部 FANCC 一抗
  • 描述
    兔多克隆抗体to FANCC
  • 特异性Detects a band at 60kDa in Hela cell lysate corresponding to FANCC.
  • 经测试应用适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Human
    不与反应: Mouse
  • 免疫原

    Synthetic peptide:

    CLINKEPQNSGSKLNS

    , corresponding to amino acids 96 - 112 of Human FANCC.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.878% Sodium chloride
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab5065 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
WB 1/500 - 1/2000. Detects a band of approximately 60 kDa (predicted molecular weight: 63 kDa).

靶标

  • 功能DNA repair protein that may operate in a postreplication repair or a cell cycle checkpoint function. May be implicated in interstrand DNA cross-link repair and in the maintenance of normal chromosome stability. Upon IFNG induction, may facilitate STAT1 activation by recruiting STAT1 to IFNGR1.
  • 组织特异性Ubiquitous.
  • 疾病相关Defects in FANCC are the cause of Fanconi anemia complementation group C (FANCC) [MIM:227645]. A disorder affecting all bone marrow elements and resulting in anemia, leukopenia and thrombopenia. It is associated with cardiac, renal and limb malformations, dermal pigmentary changes, and a predisposition to the development of malignancies. At the cellular level it is associated with hypersensitivity to DNA-damaging agents, chromosomal instability (increased chromosome breakage) and defective DNA repair.
  • 发展阶段Expression increases during S phase, is maximal at the G2/M transition, and declines during M phase (at protein level).
  • 细胞定位Nucleus. Cytoplasm. The major form is nuclear. The minor form is cytoplasmic.
  • Information by UniProt
  • 数据库链接
  • 别名
    • bA80I15.1 antibody
    • FA 3 antibody
    • FA3 antibody
    • FAC antibody
    • FACC antibody
    • FANCC antibody
    • FANCC_HUMAN antibody
    • Fanconi anemia complementation group C antibody
    • Fanconi anemia complementation group C protein antibody
    • Fanconi anemia group C protein antibody
    • Fanconi pancytopenia type 3 antibody
    • FLJ14675 antibody
    • Protein FACC antibody
    see all

Anti-FANCC antibody 图像

  • Anti-FANCC antibody (ab5065) at 1/1500 dilution + HeLa whole cell lysate at 35 µg

    Secondary
    IRDye 800 conjugated Goat anti-Rabbit IgG [H&L] at 1/10000 dilution

    Predicted band size : 63 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 33 kDa. We are unsure as to the identity of these extra bands.
  • ICC/IF image of ab5065 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab5065, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-FANCC antibody (ab5065)参考文献

This product has been referenced in:
  • Lu WT  et al. The Fanconi anemia pathway is downregulated upon macrophage differentiation through two distinct mechanisms. Cell Cycle 10: (2011). WB ; Human . Read more (PubMed: 21926477) »
  • Palagyi A  et al. Genetic inactivation of the Fanconi anemia gene FANCC identified in the hepatocellular carcinoma cell line HuH-7 confers sensitivity towards DNA-interstrand crosslinking agents. Mol Cancer 9:127 (2010). WB ; Human . Read more (PubMed: 20509860) »

See all 2 Publications for this product

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I am sorry that this antibody did not perform as stated on the datasheet. If payment has already been made on the original order and you wish to receive a refund, please ask your purchasing department to contact ...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HeLa cells)
Loading amount 30 µg
Specification HeLa cells
Gel Running Conditions Reduced Denaturing (4-20% Tris-Gly gel)
Blocking step LI-COR® Odyssey® Blocking Buffer as blocking agent for 45 minute(s) · Concentration: 50% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 May 29 2012

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (Mouse embryonic fibroblasts)
Loading amount 40 µg
Specification Mouse embryonic fibroblasts
Gel Running Conditions Reduced Denaturing (4-20% Tris-Gly gel)
Blocking step LI-COR® Odyssey® Blocking Buffer as blocking agent for 45 minute(s) · Concentration: 50% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 May 29 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (RKO)
Loading amount 20 µg
Specification RKO
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 minute(s) · Concentration: 10% · Temperature: 18°C
Username

Abcam user community

Verified customer

提交于 Mar 06 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HuH-7 lysates transduced w/ retrooncoviral vector)
Loading amount 30 µg
Specification HuH-7 lysates transduced w/ retrooncoviral vector
Gel Running Conditions Reduced Denaturing (12% Bis-Tris, MES Buffer)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Dr. Lukas Rambousek

Verified customer

提交于 May 03 2010

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (MEF)
Loading amount 30 µg
Specification MEF
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Aug 15 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (THP1 cells)
Loading amount 126 µg
Specification THP1 cells
Blocking step BSA as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 3%
Username

Dr. Wei-Ting Lu

Verified customer

提交于 May 29 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"