概述

  • 产品名称
  • 描述
    兔多克隆抗体to FANCA
  • 特异性
    Detects a band at 130kDa in HeLa cell lysate that we believe corresponds to FANCA.
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide:

    SRSYDHSENSDLVFG-C

    conjugated to KLH, corresponding to amino acids 995 - 1009 of Human FANCA.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.01% Sodium Azide
    Constituents: 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab5063 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/2000. Detects a band of approximately 130 kDa (predicted molecular weight: 163 kDa).

靶标

  • 功能
    DNA repair protein that may operate in a postreplication repair or a cell cycle checkpoint function. May be involved in interstrand DNA cross-link repair and in the maintenance of normal chromosome stability.
  • 疾病相关
    Defects in FANCA are a cause of Fanconi anemia (FA) [MIM:227650]. FA is a genetically heterogeneous, autosomal recessive disorder characterized by progressive pancytopenia, a diverse assortment of congenital malformations, and a predisposition to the development of malignancies. At the cellular level it is associated with hypersensitivity to DNA-damaging agents, chromosomal instability (increased chromosome breakage), and defective DNA repair.
  • 翻译后修饰
    Phosphorylated upon DNA damage, probably by ATM or ATR. Phosphorylation is required for the formation of the nuclear complex. Not phosphorylated in cells derived from groups A, B, C, E, F, G, and H.
  • 细胞定位
    Nucleus. Cytoplasm. The major form is nuclear. The minor form is cytoplasmic.
  • Information by UniProt
  • 数据库链接
  • 别名
    • FA 1 antibody
    • FA antibody
    • FA H antibody
    • FA1 antibody
    • FAA antibody
    • FACA antibody
    • FAH antibody
    • Fanca antibody
    • FANCA_HUMAN antibody
    • FANCH antibody
    • Fanconi anemia complementation group A antibody
    • Fanconi anemia complementation group H antibody
    • Fanconi anemia group A protein antibody
    • Fanconi anemia type 1 antibody
    • MGC75158 antibody
    • Protein FACA antibody
    see all

图片

  • Anti-FANCA antibody (ab5063) at 1/1500 dilution + HeLa whole cell lysate at 35 µg

    Secondary
    IRDye 800 conjugated Goat anti-Rabbit IgG [H&L] at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 163 kDa
    Observed band size : 130 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 42 kDa,75 kDa. We are unsure as to the identity of these extra bands.

实验方案

文献

This product has been referenced in:

See all 8 Publications for this product

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Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa)
Loading amount
50 µg
Specification
HeLa
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Aug 14 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (THP1 - Human acute monocytic leukemia cell)
Loading amount
150 µg
Specification
THP1 - Human acute monocytic leukemia cell
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Username

Dr. Wei-Ting Lu

Verified customer

提交于 Jul 24 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Cal51 & SW620)
Loading amount
750 cells
Specification
Cal51 & SW620
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10%
Username

Mr. Richard Allum

Verified customer

提交于 Oct 24 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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