概述

  • 产品名称
  • 描述
    小鼠多克隆抗体to FAN1
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length human MTMR15 protein (AAH47882, 1 a.a. ~ 533 a.a)

  • 阳性对照
    • Human spleen lysate. MTMR15 transfected 293T cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab68572 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 114 kDa (predicted molecular weight: 114 kDa).

靶标

  • 功能
    Nuclease required for maintenance of chromosomal stability. Plays a key role in DNA repair of DNA interstrand cross-links (ICL) by being recruited to sites of DNA damage by monoubiquitinated FANCD2. Specifically involved in repair of ICL-induced DNA breaks by being required for efficient homologous recombination, possibly in the resolution of homologous recombination intermediates. Not involved in DNA double-strand breaks resection. Has both endonuclease activity toward 5'-flaps and 5'-exonuclease activity: may act in concert with the 3'-flap-specific enzymes to unhook the ICL by cleaving the lagging-strand template.
  • 序列相似性
    Belongs to the FAN1 family.
    Contains 1 UBZ-type zinc finger.
    Contains 1 VRR-NUC domain.
  • 结构域
    The UBZ-type zinc finger specifically binds monoubiquitinated FANCD2.
  • 细胞定位
    Nucleus. Localizes at sites of DNA damage following recruitment by monoubiquitinated FANCD2.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 6030441H18Rik antibody
    • Coiled coil domain containing protein MTMR15 antibody
    • DKFZp451H236 antibody
    • DKFZp686K16147 antibody
    • FAN1 antibody
    • FAN1_HUMAN antibody
    • FANCD2 and FANCI associated nuclease 1 antibody
    • FANCD2/FANCI associated nuclease 1 antibody
    • FANCD2/FANCI-associated nuclease 1 antibody
    • Fanconi associated nuclease 1 antibody
    • Fanconi-associated nuclease 1 antibody
    • hFAN1 antibody
    • KIAA1018 antibody
    • KMIN antibody
    • MTMR 15 antibody
    • MTMR15 antibody
    • Myotubularin related protein 15 antibody
    • Myotubularin-related protein 15 antibody
    see all

图片

  • Anti-FAN1 antibody (ab68572) at 1/500 dilution + Human spleen lysate at 25 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 114 kDa
    Observed band size : 114 kDa
  • All lanes : Anti-FAN1 antibody (ab68572) at 1/500 dilution

    Lane 1 : MTMR15 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 114 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)

实验方案

文献

ab68572 has not yet been referenced specifically in any publications.

客户评价及客户问答

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa cell line)
Loading amount
40 µg
Specification
HeLa cell line
Gel Running Conditions
Reduced Denaturing (6%)
Blocking step
BSA as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Oct 31 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册