antigen: EEEEGEEEEL IRIFQEQQKK WQQYRSVRRE RLKEMKLLRD QFVKALEDFE DLCDRVFSDE DSEL, corresponding to amino acids 121-184 of Human FAM9B (Q8IZU0)
Human colon tissue; Human cell line U-2 OS
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide Constituents: 59% PBS, 40% Glycerol
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Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
Use a concentration of 1 - 4 µg/ml. Fixation/Permeabilization: PFA/Triton X-100
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Anti-FAM9B antibody 图像
Immunocytochemistry/ Immunofluorescence - Anti-FAM9B antibody (ab122253)
Immunofluorescent staining of Human cell line U-2 OS using ab122253 at 4 µg/ml. Note: ab122253 staining is green.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAM9B antibody (ab122253)
Immunohistochemical staining of paraffin embedded Human colon tissue using 1/500 ab122253.
Western blot - Anti-FAM9B antibody (ab122253)
Lane 1: Marker [kDa] 250; 130; 95; 72; 55; 36; 28; 17; 10
Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells.
Anti-FAM9B antibody (ab122253)参考文献
has not yet been referenced specifically in any publications.
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