Chimpanzee, Gorilla, Orangutan
Synthetic peptide, corresponding to a region within amino acids 420 - 470 of Human FAM82A2 (NP_060615.1).
HeLa whole cell lysates and 293T cell lysates
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
Constituents: Tris citrate/phosphate, pH 7 to 8
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Immunogen affinity purified
ab100803 is affinity purified using an epitope specific to FAM82A2 immobilized on solid support.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Predicted molecular weight: 52 kDa.
Use at 2-5 µg/mg of lysate.
FAM82A2, or family with sequence similarity 82, member C, may participate in differentiation and apoptosis of keratinocytes. Overexpression of FAM82A2 induces apoptosis.
Mitochondrion membrane; Single-pass membrane protein. Cytoplasm. Nucleus. Note=In interphase localizes in the cytoplasm, and during mitosis localizes to the spindle microtubules and spindle poles.
Cerebral protein 10 antibody
FAM82C, formerly antibody
Western blot - Anti-FAM82A2 antibody (ab100803)
All lanes : Anti-FAM82A2 antibody (ab100803) at 0.1 µg/ml Lane 1 : HeLa whole cell lysates at 50 µg Lane 2 : HeLa whole cell lysates at 15 µg Lane 3 : HeLa whole cell lysates at 5 µg Lane 4 : 293T cell lysates at 50 µg Developed using the ECL technique. Predicted band size: 52 kDa Exposure time: 3 minutes
Immunoprecipitation - Anti-FAM82A2 antibody (ab100803)
Detection of Human FAM82A2 by western blot of immunoprecipitates of HeLa whole cell lysates (at 1 mg for IP, 20% of IP loaded) using ab100803 at 3 µg/mg lysate for immunoprecipitation and at 1 µg/ml for subsequent blotting.
Detection by chemiluminescence with an exposure time of 30 seconds.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"