antigen sequence, corresponding to internal sequence amino acids 279-405 of Human FAM73A.
- Human small intestine tissue; Human tonsil and liver tissue lysates; RT-4 and U-251 MG cell lysates.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
Immunogen affinity purified
Our Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
||1/10 - 1/20. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
||1/250 - 1/500.
Anti-FAM73A antibody 图像
Immunocytochemistry/ Immunofluorescence - Anti-FAM73A antibody (ab121532)
Immunofluorescent staining of Human cell line A-431 shows positivity in mitochondria. Recommended concentration of ab121532 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAM73A antibody (ab121532)
ab121532 at 1/10 dilution staining FAM73A in paraffin-embedded Human small intestine tissue by Immunohistochemistry.
Western blot - Anti-FAM73A antibody (ab121532)
All lanes : Anti-FAM73A antibody (ab121532) at 1/250 dilution
Lane 1 : RT-4 cell lysate
Lane 2 : U-251 MG cell lysate
Lane 3 : Human Plasma
Lane 4 : Human liver tissue lysate
Lane 5 : Human tonsil tissue lysate
Developed using the ECL technique
Anti-FAM73A antibody (ab121532)参考文献
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"