概述

  • 产品名称Anti-FAM71C抗体
    参阅全部 FAM71C 一抗
  • 描述
    兔多克隆抗体to FAM71C
  • 经测试应用适用于: IHC-P, WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    antigen sequence: YILRPPVEAY SDTRAILAGN TLDSSVLEEV QRSPVGYAMK FCEEKEQFRI SRLHMNAEMF GSTYCDYTIE, corresponding to amino acids of Human FAM71C.

  • 阳性对照
    • Human colon tissue.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

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应用

Our Abpromise guarantee covers the use of ab122799 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use at an assay dependent concentration.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

靶标

Anti-FAM71C antibody 图像

  • Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus but not nucleoli and cytoskeleton (microtubules). Recommended concentration of ab122799 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • Lane 1: Marker [kDa] 250; 130; 95; 72; 55; 36; 28; 17; 10
    Lane 2: Negative control (vector only transfected HEK293T lysate)
    Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells.
  • ab122799, at 1/50 dilution, staining FAM71C in Paraffin-embedded Human colon tissue by Immunohistochemistry

Anti-FAM71C antibody (ab122799)参考文献

ab122799 has not yet been referenced specifically in any publications.

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