antigen sequence, corresponding to internal sequence amino acids 62-196 of Human FAM69A, isoform 1.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Belongs to the FAM69 family.
Among the many cysteines in the lumenal domain, most are probably involved in disulfide bonds.
Endoplasmic reticulum membrane.
Information by UniProt
- FA69A_HUMAN antibody
- fam69a antibody
- Family with sequence similarity 69, member A antibody
- Protein FAM69A antibody
Immunocytochemistry/ Immunofluorescence - Anti-FAM69A antibody (ab121529)
Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus and cytoplasm. Recommended concentration of ab121529 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAM69A antibody (ab121529)
ab121529 at 1/125 dilution staining FAM69A in Human duodenum by Immunohistochemistry.
has not yet been referenced specifically in any publications.
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