Anti-FAM3C抗体(ab72182)

概述

  • 产品名称Anti-FAM3C抗体
    参阅全部 FAM3C 一抗
  • 描述
    兔多克隆抗体to FAM3C
  • 经测试应用适用于: IHC-Fr, WB, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Chicken, Cow, Dog, Human, Monkey
    预测可用于: Mouse, Rat, Chimpanzee, Pufferfish (Fugu), Opossum
  • 免疫原

    Synthetic peptide corresponding to a portion of amino acids 40-80 of Human FAM3C

  • 阳性对照
    • Human, mouse and rat brain lysates. Human brain and skin tissue.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab72182 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr Use at an assay dependent concentration.
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 25 kDa).
IHC-P Use a concentration of 5 - 10 µg/ml.
ICC/IF Use a concentration of 5 µg/ml.

靶标

Anti-FAM3C antibody 图像

  • All lanes : Anti-FAM3C antibody (ab72182) at 0.5 µg/ml

    Lane 1 : Human brain lysate
    Lane 2 : Human brain lysate with immunising peptide
    Lane 3 : Mouse brain lysate
    Lane 4 : Rat brain lysate


    Predicted band size : 25 kDa
    Observed band size : 30 kDa (why is the actual band size different from the predicted?)
  • ab72182 at 5µg/ml staining FAM3C in normal human brain by Immunohistochemistry using paraffin-embedded tissue. (Control rabbit Ig used for figure B).
  • ab72182 at 10µg/ml staining FAM3C in human skin by Immunohistochemistry using paraffin-embedded tissue.
  • ICC/IF image of ab72182 stained SKNSH cells. The cells were 4% PFA fixed (10mins) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab72182, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-FAM3C antibody (ab72182)参考文献

ab72182 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (Prostate)
Specification Prostate
Fixative Methanol
Permeabilization No
Blocking step BSA as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
Username

Abcam user community

Verified customer

提交于 Jun 08 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (prostate)
Specification prostate
Fixative Paraformaldehyde
Permeabilization No
Blocking step BSA as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
Username

Dr. Olayinka Oloko

Verified customer

提交于 Dec 08 2011

Application Western blot
Sample Mouse Cell lysate - whole cell (Mouse mammary cell)
Loading amount 50 µg
Specification Mouse mammary cell
Treatment 5ng/ml TGF beta for different time periods (hrs)
Gel Running Conditions Reduced Denaturing (10.0% gel)
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

提交于 Aug 31 2011

Application Immunocytochemistry
Sample Human Cell (Prostate)
Specification Prostate
Fixative Methanol
Permeabilization Yes - Triton X-100
Blocking step BSA as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
Username

Dr. Olayinka Oloko

Verified customer

提交于 May 27 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Prostate)
Loading amount 25 µg
Specification Prostate
Gel Running Conditions Non-reduced Non-Denaturing (Native) (10% gel)
Blocking step Milk as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 4°C
Username

Dr. Olayinka Oloko

Verified customer

提交于 Apr 01 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"