Anti-FAM3B抗体(ab88142)

概述

性能

应用

Our Abpromise guarantee covers the use of ab88142 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 26 kDa.
ELISA Use at an assay dependent dilution.

靶标

  • 功能Induces apoptosis of alpha and beta cells in a dose- and time-dependent manner. Present in insulin secretory granules and likely cosecreted with insulin.
  • 组织特异性Highly expressed in the pancreas. Also found in the colon, kidney, prostate, small intestine and testis.
  • 序列相似性Belongs to the FAM3 family.
  • 翻译后修饰2 N-termini have been observed in the mature protein: the first at Glu-30, resulting from signal peptide cleavage, the second at Ser-46.
  • 细胞定位Secreted. Localized in discrete vesicular and perinuclear structure.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 2-21 antibody
    • C21orf11 antibody
    • C21orf76 antibody
    • Cytokine like protein 2 21 antibody
    • Cytokine-like protein 2-21 antibody
    • Fam3b antibody
    • FAM3B_HUMAN antibody
    • Family with sequence similarity 3 member B antibody
    • ORF9 antibody
    • Pancreatic-derived factor antibody
    • PANDER antibody
    • PRED44 antibody
    • Protein FAM3B antibody
    see all

Anti-FAM3B antibody 图像

  • Anti-FAM3B antibody (ab88142) at 1 µg/ml + immunogen (recombinant full length protein with proprietary tag of 26kDa) at 0.2 µg

    Secondary
    Goat anti-Mouse IgG at 1/5000 dilution

    Predicted band size : 26 kDa
  • Anti-FAM3B antibody (ab88142) at 5 µg/ml + Human kidney tissue lysate at 25 µg

    Secondary
    Goat anti-Mouse IgG at 1/2500 dilution

    Predicted band size : 26 kDa
  • All lanes : Anti-FAM3B antibody (ab88142) at 5 µg/ml

    Lane 1 : FAM3B-transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Lane 1 : Goat anti-Mouse IgG at 1/2500 dilution
    Lane 2 : Goat anti-Mouse IgG at 1/2500 dilution


    Predicted band size : 26 kDa

Anti-FAM3B antibody (ab88142)参考文献

ab88142 has not yet been referenced specifically in any publications.

Product Wall

Thank you for sending your customer's protocol.

It is mostly correct, but for 293T cells, the antibody needs to be used at a concentration of at least 5ug/ml (1/200) in order to develop even a weak signal. The datasheet shows an image of a 2...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"