概述

  • 产品名称
  • 描述
    兔多克隆抗体to FAM38B
  • 特异性
    Specific to isoforms 1, 2, 3 and 4.
  • 经测试应用
    适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Dog
  • 免疫原

    Synthetic peptide: AGNSTESSKT PVTIEKIYPY YVKAPSDSNS KPIKQLLSEN NFMDITIILS, corresponding to amino acids 2503-2552 of Human FAM38B

  • 阳性对照
    • 721_B cell lysate.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab81327 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 318 kDa.

Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/312500.

靶标

图片

  • Anti-FAM38B antibody (ab81327) at 1 µg/ml (in 5% skim milk / PBS buffer) + 721_B cell lysate at 10 µg

    Secondary
    HRP conjugated anti Rabbit IgG at 1/50000 dilution

    Predicted band size : 318 kDa
    Observed band size : 95 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 70 kDa. We are unsure as to the identity of these extra bands.

    Isoform 3 has predicted MW 81 kDa.

    Gel concentration: 12%

实验方案

文献

ab81327 has not yet been referenced specifically in any publications.

客户评价及客户问答

There are currently no Customer reviews or Questions for ab81327.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册