概述

  • 产品名称Anti-FAM21C抗体
    参阅全部 FAM21C 一抗
  • 描述
    小鼠多克隆抗体to FAM21C
  • 经测试应用适用于: WB, IP, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length human FAM21C protein (AAH06456, 1 a.a. ~ 283 a.a)

  • 阳性对照
    • FAM21C transfected 293T cell lysate. IHC-P: FFPE human lung tissue sections.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: Whole serum
  • 纯度Whole antiserum
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab72720 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 35 kDa (predicted molecular weight: 31 kDa).
IP Use at an assay dependent concentration. PubMed: 20923837
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能Component of the WASH complex, a complex present at the surface of endosomes that recruits and activates the Arp2/3 complex to induce actin polymerization. The WASH complex plays a key role in the fission of tubules that serve as transport intermediates during endosome sorting. In the complex, it probably mediates the recruitment of the complex to endosome membranes. Plays a role in fluid-phase endocytosis, a process exploited by vaccinia intracellular mature virus (IMV) to enter cells. As a result, may facilitate the penetration of IMV into cells.
  • 序列相似性Belongs to the FAM21 family.
  • 细胞定位Early endosome membrane. Cell membrane. Partially colocalizes with RAB11A, a recycling endosome marker. Associates with lipid raft microdomains on the plasma membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • FA21C_HUMAN antibody
    • FAM21A antibody
    • FAM21C antibody
    • Family with sequence similarity 21 member C antibody
    • Hypothetical protein LOC253725 antibody
    • KIAA0592 antibody
    • Protein FAM21C antibody
    • Vaccinia virus penetration factor antibody
    • VPEF antibody
    • WASH complex subunit FAM21C antibody
    see all

Anti-FAM21C antibody 图像

  • IHC image of FAM21C staining in human lung formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab72720, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • All lanes : Anti-FAM21C antibody (ab72720) at 1/500 dilution

    Lane 1 : FAM21C transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 31 kDa
    Observed band size : 35 kDa (why is the actual band size different from the predicted?)
  • ab72720 used in Immunoprecipitation.

Anti-FAM21C antibody (ab72720)参考文献

This product has been referenced in:
  • Harbour ME  et al. The cargo-selective retromer complex is a recruiting hub for protein complexes that regulate endosomal tubule dynamics. J Cell Sci 123:3703-17 (2010). IP ; Human . Read more (PubMed: 20923837) »

See 1 Publication for this product

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