antigen sequence: AVKKEEKCVP PYVQIRDLHG ILRTYANFSI TKELKDTMRT SHGLRRHPSF SANCGLPSSW TSTWQVADDL TQNTLDLEYL RFAHKLKQTI KNGDSQHSA, corresponding to internal sequence amino acids 1901-1999 of Human FAM208B protein (UniProt ID: Q5VWN6).
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.02% Sodium azide Constituents: 59% PBS, 40% Glycerol
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
Information by UniProt
Uncharacterized protein C10orf18 antibody
Immunocytochemistry/ Immunofluorescence - Anti-FAM208B antibody (ab122433)
Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus but not nucleoli, plasma membrane and cytoplasm. Recommended concentration of ab122433 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAM208B antibody (ab122433)
ab122433, at a 1/50 dilution, staining FAM208B in paraffin embedded Human rectum tissue by Immunohistochemistry.
has not yet been referenced specifically in any publications.
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