概述

  • 产品名称Anti-FAM199X抗体
  • 描述
    兔多克隆抗体to FAM199X
  • 经测试应用适用于: WB, ICC/IF, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment, corresponding to amino acids 181-309 of Human FAM199X.

  • 阳性对照
    • Human cerebral cortex tissue; RT4 lysate.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at -20ºC.
  • 存储溶液pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab121167 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent concentration.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/10 - 1/20. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use buffer at pH 6 for antigen retrieval.

靶标

Anti-FAM199X antibody 图像

  • Immunofluorescent staining of Human cell line U-251MG shows positivity in nucleus but not nucleoli, cytoplasm and golgi apparatus. Recommended concentration of ab121167 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab121167 at 1/10 dilution staining FAM199X in Paraffin-emebedded Human cerebral cortex tissue by Immunohistochemistry.

    Note: strong nuclear and moderate cytoplasmic positivity in neuronal cells.
  • All lanes : Anti-FAM199X antibody (ab121167) at 1/250 dilution

    Lane 1 : RT4 lysate
    Lane 2 : U251 MG lysate
    Lane 3 : Human plasma
    Lane 4 : Human liver lysate
    Lane 5 : Human tonsil lysate

    Developed using the ECL technique

Anti-FAM199X antibody (ab121167)参考文献

ab121167 has not yet been referenced specifically in any publications.

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