Recombinant fragment, corresponding to amino acids 181-309 of Human FAM199X.
Human cerebral cortex tissue; RT4 lysate.
Shipped at 4°C. Store at -20ºC.
Preservative: 0.02% Sodium azide Constituents: 59% PBS, 40% Glycerol
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
1/10 - 1/20. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use buffer at pH 6 for antigen retrieval.
Belongs to the FAM199 family.
Information by UniProt
Immunocytochemistry/ Immunofluorescence - Anti-FAM199X antibody (ab121167)
Immunofluorescent staining of Human cell line U-251MG shows positivity in nucleus but not nucleoli, cytoplasm and golgi apparatus. Recommended concentration of ab121167 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAM199X antibody (ab121167)
ab121167 at 1/10 dilution staining FAM199X in Paraffin-emebedded Human cerebral cortex tissue by Immunohistochemistry.
Note: strong nuclear and moderate cytoplasmic positivity in neuronal cells.
Western blot - Anti-FAM199X antibody (ab121167)
All lanes : Anti-FAM199X antibody (ab121167) at 1/250 dilution Lane 1 : RT4 lysate Lane 2 : U251 MG lysate Lane 3 : Human plasma Lane 4 : Human liver lysate Lane 5 : Human tonsil lysate Developed using the ECL technique
has not yet been referenced specifically in any publications.
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