概述

  • 产品名称
    Anti-FAM190B抗体
    参阅全部 FAM190B 一抗
  • 描述
    兔多克隆抗体to FAM190B
  • 经测试应用
    适用于: ICC/IF, IHC-P, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    internal sequence amino acids 568-664 (NVECDNMNRF DRPDRNVRQP QEGFWKRPPQ RWSGQEHYHL SHPDHYHHHG KSDLSRGSPY RESPLGHFES YGGMPFFQAQ KMFVDVPENT VILDEMT) of the Human FAM190B protein (Q9H7U1).

  • 阳性对照
    • IHC-P: Human adrenal gland tissue WB: RT4, U251 MG, Human Liver and Tonsil cell lysates (weak bands)

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab122393 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.

靶标

图片

  • Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus but not nucleoli and cytoplasm. Recommended concentration of ab122393 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)
    Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)
  • All lanes : Anti-FAM190B antibody (ab122393) at 1/250 dilution

    Lane 1 : RT4 cell lysate
    Lane 2 : U251 MG cell lysate
    Lane 3 : Human Plasma cell lysate
    Lane 4 : Liver cell lysate
    Lane 5 : Tonsil cell lysate
  • ab122393, at 1/500, staining FAM190B in paraffin embedded adrenal gland tissue by immunohistochemistry.

文献

ab122393 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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