Anti-FAM184A抗体(ab122215)

概述

  • 产品名称Anti-FAM184A抗体
  • 描述
    兔多克隆抗体to FAM184A
  • 经测试应用适用于: IHC-P, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    antigen sequence, corresponding to amino acids 262-365 of Human FAM184A.

  • 阳性对照
    • Human heart muscle tissue; HEK293T cell FAM184A over-expression lysate.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab122215 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.

靶标

Anti-FAM184A antibody 图像

  • ab122215, at 1/300 dilution, staining FAM184A in paraffin-embedded Human heart muscle tissue by Immunohistochemistry.
  • All lanes : Anti-FAM184A antibody (ab122215) at 1/250 dilution

    Lane 1 : Negative control (vector only transfected HEK293T lysate)
    Lane 2 : FAM184A over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells

    Developed using the ECL technique
  • Immunohistochemical analysis of Human umbilical vein endothelial cells, staining FAM184A with ab122215.

    Tissue was fixed with paraformaldehyde, permeabilized with Triton X-100 and blocked with 2% BSA for 45 minutes at 25°. Samples were incubated with primary antibody (5 µg/ml in diluent) for 1 hour at 25°C. A Cy3®-conjugated donkey anti-rabbit polyclonal IgG (1/1000) was used as the secondary antibody.

    See Abreview

Anti-FAM184A antibody (ab122215)参考文献

ab122215 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (human umbilical vein endothelial cells)
Specification human umbilical vein endothelial cells
Fixative Paraformaldehyde
Permeabilization Yes - triton X
Blocking step BSA as blocking agent for 45 minute(s) · Concentration: 2% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Nov 01 2012

Abreviews
Application Western blot
Sample Human Cell lysate - whole cell (HeLa and RPE-1)
Loading amount 50 µg
Specification HeLa and RPE-1
Gel Running Conditions Reduced Denaturing (4-12% Bis-Tris (MES Buffer))
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

提交于 Oct 10 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"