antigen, corresponding to C-terminus amino acids 232-352 of the Human FAM181A protein (Q8N9Y4).
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/100 - 1/250.
||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use buffer at pH 6 for antigen retrieval.
Belongs to the FAM181 family.
Information by UniProt
- C14orf152 antibody
- F181A_HUMAN antibody
- FAM181A antibody
Immunocytochemistry/ Immunofluorescence - Anti-FAM181A antibody (ab121144)
Immunofluorescent staining of Human cell line A-431 shows positivity in nucleoli. Recommended concentration of ab121144 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Western blot - Anti-FAM181A antibody (ab121144)
Lanes 2-3 : Anti-FAM181A antibody (ab121144)
Lane 1 : Marker
Lane 2 : HEK293T cell lysate - vector only transfected
Lane 3 : HEK293T cell lysate - overexpressing FAM181A with a C-terminal myc-DDK tag (~3.1kDa)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAM181A antibody (ab121144)
Immunohistochemical staining of Human testis with ab121144 at 1/100 dilution
has not yet been referenced specifically in any publications.
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