antigen sequence: RRNRKTRRYG VLDTNIENME LTPLEQDDED DDNTLFDANH PRR, corresponding to C terminal amino acids 148-190 of the Human FAM174A protein.
- Human hippocampus tissue; Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag in mammalian HEK293T cells.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
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Immunogen affinity purified
Our Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
||1/250 - 1/500.
Anti-FAM174A antibody 图像
Immunocytochemistry/ Immunofluorescence - Anti-FAM174A antibody (ab121607)
Immunofluorescent staining of Human cell line U-251MG shows positivity in golgi apparatus. Recommended concentration of ab121607 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAM174A antibody (ab121607)
ab121607 at 1/120 dilution staining FAM174A in Paraffin-embedded Human hippocampus tissue by Immunohistochemistry.
Note: strong cytoplasmic positivity with a granular pattern in neuronal cells.
Western blot - Anti-FAM174A antibody (ab121607)
All lanes : Anti-FAM174A antibody (ab121607) at 1/250 dilution
Lane 1 : Negative control (vector only transfected HEK293T lysate)
Lane 2 : Over-expression Lysate [Co-expressed with a C-terminal proprietary tag (~3.1 kDa)] in mammalian HEK293T cells.
Developed using the ECL technique
Anti-FAM174A antibody (ab121607)参考文献
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"