ICC/IF, IHC-P more details
antigen, corresponding to amino acids 135-162 (HESLTSWNLAKKAKWREEAALAAQAKAK) of Human FAM162B.
存放说明 Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
存储溶液 pH: 7.20 Preservative: 0.02% Sodium azide Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
纯度 Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
1/750. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
序列相似性 Belongs to the UPF0389 family.
Information by UniProt
Protein FAM162B antibody
Anti-FAM162B antibody 图像
Immunocytochemistry/ Immunofluorescence - Anti-FAM162B antibody (ab122309)
Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus but not nucleoli and cytoplasm. Recommended concentration of ab122309 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAM162B antibody (ab122309)
ab122309, at 1/750, staining FAM162B in paraffin embedded Human testis tissue by Immunohistochemistry.
Anti-FAM162B antibody (ab122309)参考文献
This product has been referenced in:
et al. Male-biased aganglionic megacolon in the TashT mouse line due to perturbation of silencer elements in a large gene desert of chromosome 10. PLoS Genet 11:e1005093 (2015).
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