antigen sequence: MAASAGGPGS WSENILEYFL RNSQITAEDG AEITWYHAAN HKAQTNEALK STAHMIEADV LLPSDGSEHS QPIMAHPPET NSDNTLQEWL TEVMK, corresponding to N terminal amino acids 1-95 of Human FAM151B.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide Constituents: 59% PBS, 40% Glycerol
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Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Use at an assay dependent concentration.
Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
Anti-FAM151B antibody 图像
Immunocytochemistry/ Immunofluorescence - Anti-FAM151B antibody (ab122863)
Immunofluorescent staining of Human cell line U-2 OS shows positivity in cytoplasm. Recommended concentration of ab122863 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Western blot - Anti-FAM151B antibody (ab122863)
Lane 1: Marker [kDa] 250; 130; 95; 72; 55; 36; 28; 17; 10
Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAM151B antibody (ab122863)
ab122863 at 1/50 dilution staining FAM151B in paraffin-embedded Human colon tissue by Immunohistochemistry.
Anti-FAM151B antibody (ab122863)参考文献
has not yet been referenced specifically in any publications.
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