antigen sequence, corresponding to amino acids 620-707 (ISQDDSEITQ MEHNLASRRS SDDCHDHQTT PSLGVRTIEI KPSNKDPFSG ENITVKLGPW TELRQEEILV DNLLPNFESL ESNGKSKS) of Human FAM135A.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Anti-FAM135A antibody 图像
Immunocytochemistry/ Immunofluorescence - Anti-FAM135A antibody (ab122191)
Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus but not nucleoli and cytoplasm. Recommended concentration of ab122191 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAM135A antibody (ab122191)
ab122191 at 1/250 dilution staining FAM135A in Paraffin-embedded Human Testis tissue by Immunohistochemistry.
Anti-FAM135A antibody (ab122191)参考文献
has not yet been referenced specifically in any publications.
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