antigen sequence, corresponding to amino acids 1148-1230 (CTSGCLSFPS APRESPCNVK YSSKSKFDAI TKQPSSTSYN FTSSISWYES SPKPQIQAFL QAKEELKLLK LPGFMYSEVP LLA) of Human FAM135A.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
||1/10 - 1/20. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Anti-FAM135A antibody 图像
Immunocytochemistry/ Immunofluorescence - Anti-FAM135A antibody (ab122190)
Immunofluorescent staining of Human cell line U-251MG shows positivity in nucleus but not nucleoli. Recommended concentration of ab122190 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAM135A antibody (ab122190)
ab122190 at 1/15 dilution staining FAM135A in Paraffin-embedded Human Kidney tissue by Immunohistochemistry.
Anti-FAM135A antibody (ab122190)参考文献
has not yet been referenced specifically in any publications.
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