Anti-FAK (phospho Y925)抗体(ab38512)


  • 产品名称
    Anti-FAK (phospho Y925)抗体
    参阅全部 FAK 一抗
  • 描述
    兔多克隆抗体to FAK (phospho Y925)
  • 宿主
  • 特异性
    This antibody is specific for FAK only when phosphorylated at tyrosine 925.
  • 经测试应用
    适用于: WB, ELISA, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic phosphopeptide derived from Human FAK around the phosphorylation site of tyrosine 925 (KVYpEN).

  • 阳性对照
    • HepG2 and 293 cell extract.



Our Abpromise guarantee covers the use of ab38512 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 123 kDa (predicted molecular weight: 119 kDa).
ELISA 1/4000.
ICC/IF Use at an assay dependent concentration. PubMed: 22102809


  • 功能
    Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Microtubule-induced dephosphorylation at Tyr-397 is crucial for the induction of focal adhesion disassembly. Plays a potential role in oncogenic transformations resulting in increased kinase activity.
  • 组织特异性
    Expressed in all organs tested, in lymphoid cell lines, but most abundantly in brain.
  • 序列相似性
    Belongs to the protein kinase superfamily. Tyr protein kinase family. FAK subfamily.
    Contains 1 FERM domain.
    Contains 1 protein kinase domain.
  • 结构域
    The first Pro-rich domain interacts with the SH3 domain of CRK-associated substrate (BCAR1) and CASL.
    The carboxy-terminal region is the site of focal adhesion targeting (FAT) sequence which mediates the localization of FAK1 to focal adhesions.
  • 翻译后修饰
    Phosphorylated on 6 tyrosine residues upon activation. Microtubule-induced dephosphorylation at Tyr-397 could be catalyzed by PTPN11 and regulated by ZFYVE21. Dephosphorylated by PTPN11 upon EPHA2 activation by its ligand EFNA1.
  • 细胞定位
    Cell junction > focal adhesion. Cell membrane. Constituent of focal adhesions.
  • Information by UniProt
  • 数据库链接
  • 别名
    • FADK 1 antibody
    • FADK antibody
    • FAK related non kinase polypeptide antibody
    • FAK1 antibody
    • FAK1_HUMAN antibody
    • Focal adhesion kinase 1 antibody
    • Focal adhesion Kinase antibody
    • Focal adhesion kinase isoform FAK Del33 antibody
    • Focal adhesion kinase related nonkinase antibody
    • FRNK antibody
    • p125FAK antibody
    • pp125FAK antibody
    • PPP1R71 antibody
    • Protein phosphatase 1 regulatory subunit 71 antibody
    • Protein tyrosine kinase 2 antibody
    • Protein-tyrosine kinase 2 antibody
    • Ptk2 antibody
    • PTK2 protein tyrosine kinase 2 antibody
    see all


  • All lanes : Anti-FAK (phospho Y925) antibody (ab38512) at 1/500 dilution

    Lane 1 : HepG2 cell lysate, pre-incubated with phosphopeptide.
    Lane 2 : HepG2 cell lysate.
    Lane 3 : 293 cell lysate + EGF + serum.

    Lysates/proteins at 30 µg per lane.

    All lanes : Alkaline Phosphatase AffiniPure Goat Anti-Rabbit IgG (H+L).

    Predicted band size: 119 kDa
    Observed band size: 123 kDa (why is the actual band size different from the predicted?)

    Lanes can be loaded with 5-30µg of total protein.
  • ab38512 staining FAK (phospho Y925) in murine bone marrow-derived dendritic cells by Immunocytochemistry/ Immunofluorescence.

    Cells were fixed with formaldehyde and blocked with 3% BSA before being incubated with primary antibody. An AlexaFluor®568-conjugated goat anti-rabbit polyclonal IgG was used as the secondary antibody.


This product has been referenced in:
  • Gill MB  et al. KSHV-TK is a tyrosine kinase that disrupts focal adhesions and induces Rho-mediated cell contraction. EMBO J 34:448-65 (2015). Read more (PubMed: 25471072) »
  • Bernusso VA  et al. Imatinib restores VASP activity and its interaction with Zyxin in BCR-ABL leukemic cells. Biochim Biophys Acta 1853:388-95 (2015). Read more (PubMed: 25450971) »

See all 5 Publications for this product


I am still waiting for the alignment result for ab131349, but our source for ab38512 tells us that it is "designed to react with human FAK phosphorylated at Y925, mouse FAK phosphorylated at Y963, and rat FAK phosphorylated at Y928. It does not react w...

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Thank you for your enquiry. I am sorry to hear you are having a problem with ab38512 (FAK (phospho Y925) antibody). Along with some questions, I would like to suggest the following modifications to your protocol to help increase the detection of FA...

Read More