RabMAb

Anti-FAK (phospho Y861)抗体[EP841Y] (ab81293)

概述

  • 产品名称Anti-FAK (phospho Y861)抗体[EP841Y]
    参阅全部 FAK 一抗
  • 描述
    兔单克隆抗体[EP841Y] to FAK (phospho Y861)
  • 经测试应用适用于: WBmore details
    不适用于: Flow Cyt,ICC,IHC-P or IP
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to residues surrounding tyrosine 861 of human FAK

  • 阳性对照
    • Hela cell lysates treated with pervanadate.
  • 常规说明

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

应用

Our Abpromise guarantee covers the use of ab81293 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/10000 - 1/50000. Predicted molecular weight: 119 kDa.
  • 应用说明Is unsuitable for Flow Cyt,ICC,IHC-P or IP.
  • 靶标

    • 功能Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Microtubule-induced dephosphorylation at Tyr-397 is crucial for the induction of focal adhesion disassembly. Plays a potential role in oncogenic transformations resulting in increased kinase activity.
    • 组织特异性Expressed in all organs tested, in lymphoid cell lines, but most abundantly in brain.
    • 序列相似性Belongs to the protein kinase superfamily. Tyr protein kinase family. FAK subfamily.
      Contains 1 FERM domain.
      Contains 1 protein kinase domain.
    • 结构域The first Pro-rich domain interacts with the SH3 domain of CRK-associated substrate (BCAR1) and CASL.
      The carboxy-terminal region is the site of focal adhesion targeting (FAT) sequence which mediates the localization of FAK1 to focal adhesions.
    • 翻译后修饰Phosphorylated on 6 tyrosine residues upon activation. Microtubule-induced dephosphorylation at Tyr-397 could be catalyzed by PTPN11 and regulated by ZFYVE21. Dephosphorylated by PTPN11 upon EPHA2 activation by its ligand EFNA1.
    • 细胞定位Cell junction > focal adhesion. Cell membrane. Constituent of focal adhesions.
    • Information by UniProt
    • 数据库链接
    • 别名
      • FADK 1 antibody
      • FADK antibody
      • FAK related non kinase polypeptide antibody
      • FAK1 antibody
      • FAK1_HUMAN antibody
      • Focal adhesion kinase 1 antibody
      • Focal adhesion Kinase antibody
      • Focal adhesion kinase isoform FAK Del33 antibody
      • Focal adhesion kinase related nonkinase antibody
      • FRNK antibody
      • p125FAK antibody
      • pp125FAK antibody
      • PPP1R71 antibody
      • Protein phosphatase 1 regulatory subunit 71 antibody
      • Protein tyrosine kinase 2 antibody
      • Protein-tyrosine kinase 2 antibody
      • Ptk2 antibody
      • PTK2 protein tyrosine kinase 2 antibody
      see all

    Anti-FAK (phospho Y861) antibody [EP841Y] 图像

    • All lanes : Anti-FAK (phospho Y861) antibody [EP841Y] (ab81293) at 1/50000 dilution

      Lane 1 : HeLa cell lysates treated with pervanadate
      Lane 2 : HeLa cell lysates, untreated

      Secondary
      HRP goat ant-rabbit IgG (H+L) at 1/2000 dilution

      Predicted band size : 119 kDa
      Observed band size : 125 kDa (why is the actual band size different from the predicted?)


      Exposure time : 30 seconds

      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    Anti-FAK (phospho Y861) antibody [EP841Y] (ab81293)参考文献

    ab81293 has not yet been referenced specifically in any publications.

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