概述

  • 产品名称
    Anti-FAK (phospho Y861)抗体
    参阅全部 FAK 一抗
  • 描述
    兔多克隆抗体to FAK (phospho Y861)
  • 特异性
    ab38458 recognises endogenous levels of FAK only when phosphorylated at tyrosine 861.
  • 经测试应用
    适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic phosphopeptide derived from human FAK around the phosphorylation site of tyrosine 861 (HIYpQP).

  • 阳性对照
    • HepG2, HeLa and 293 cell lysate.
  • 常规说明


    Tyr861 of Focal Adhesion Kinase is a major Src phosphorylation site. Tyrosine 397 (Tyr397) is the sole autophosphorylation site of FAK. Increased levels of phosphorylated Tyr397 (P-Tyr397) thus correlate well with FAK activation. Recent studies have shown that FAK phosphorylation at Tyr861 promotes both FAK binding to alpha(v)beta5 integrin and Tyr397 phosphorylation.

性能

应用

Our Abpromise guarantee covers the use of ab38458 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Predicted molecular weight: 119 kDa.
ELISA 1/20000.

靶标

  • 功能
    Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Microtubule-induced dephosphorylation at Tyr-397 is crucial for the induction of focal adhesion disassembly. Plays a potential role in oncogenic transformations resulting in increased kinase activity.
  • 组织特异性
    Expressed in all organs tested, in lymphoid cell lines, but most abundantly in brain.
  • 序列相似性
    Belongs to the protein kinase superfamily. Tyr protein kinase family. FAK subfamily.
    Contains 1 FERM domain.
    Contains 1 protein kinase domain.
  • 结构域
    The first Pro-rich domain interacts with the SH3 domain of CRK-associated substrate (BCAR1) and CASL.
    The carboxy-terminal region is the site of focal adhesion targeting (FAT) sequence which mediates the localization of FAK1 to focal adhesions.
  • 翻译后修饰
    Phosphorylated on 6 tyrosine residues upon activation. Microtubule-induced dephosphorylation at Tyr-397 could be catalyzed by PTPN11 and regulated by ZFYVE21. Dephosphorylated by PTPN11 upon EPHA2 activation by its ligand EFNA1.
  • 细胞定位
    Cell junction > focal adhesion. Cell membrane. Constituent of focal adhesions.
  • Information by UniProt
  • 数据库链接
  • 别名
    • FADK 1 antibody
    • FADK antibody
    • FAK related non kinase polypeptide antibody
    • FAK1 antibody
    • FAK1_HUMAN antibody
    • Focal adhesion kinase 1 antibody
    • Focal adhesion Kinase antibody
    • Focal adhesion kinase isoform FAK Del33 antibody
    • Focal adhesion kinase related nonkinase antibody
    • FRNK antibody
    • p125FAK antibody
    • pp125FAK antibody
    • PPP1R71 antibody
    • Protein phosphatase 1 regulatory subunit 71 antibody
    • Protein tyrosine kinase 2 antibody
    • Protein-tyrosine kinase 2 antibody
    • Ptk2 antibody
    • PTK2 protein tyrosine kinase 2 antibody
    see all

图片

  • All lanes : Anti-FAK (phospho Y861) antibody (ab38458) at 1/500 dilution

    Lane 1 : HepG2 cells lysate pretreated with phosphopeptide (negative control).
    Lane 2 : Untreated HepG2 cell lysate
    Lane 3 : Untreated HeLa cell lysate
    Lane 4 : EGF treated 293 cell lysate.

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 119 kDa
    Observed band size : 119 kDa

实验方案

文献

This product has been referenced in:
  • Bernusso VA  et al. Imatinib restores VASP activity and its interaction with Zyxin in BCR-ABL leukemic cells. Biochim Biophys Acta 1853:388-95 (2015). Read more (PubMed: 25450971) »
  • Lal H  et al. Stretch-induced MAP kinase activation in cardiac myocytes: differential regulation through beta1-integrin and focal adhesion kinase. J Mol Cell Cardiol 43:137-47 (2007). Read more (PubMed: 17583725) »

See all 2 Publications for this product

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