RabMAb

Anti-FAK (phospho Y576 + Y577)抗体[EP1832Y] (ab76244)

概述

  • 产品名称Anti-FAK (phospho Y576 + Y577)抗体[EP1832Y]
    参阅全部 FAK 一抗
  • 描述
    兔单克隆抗体[EP1832Y] to FAK (phospho Y576 + Y577)
  • 特异性Detects FAK phosphorylated at Y576/577.
  • 经测试应用适用于: WB, IP, ICC, Flow Cytmore details
    不适用于: IHC-P
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    A phospho specific peptide corresponding to residues surrounding Tyrosine 576/577 of human FAK.

  • 阳性对照
    • NIH-3T3 lysate treated with pervanadate.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

应用

Our Abpromise guarantee covers the use of ab76244 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/50000 - 1/100000. Predicted molecular weight: 119 kDa.
IP 1/40.
ICC 1/50 - 1/100.
Flow Cyt 1/20.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • 应用说明Is unsuitable for IHC-P.
  • 靶标

    • 功能Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Microtubule-induced dephosphorylation at Tyr-397 is crucial for the induction of focal adhesion disassembly. Plays a potential role in oncogenic transformations resulting in increased kinase activity.
    • 组织特异性Expressed in all organs tested, in lymphoid cell lines, but most abundantly in brain.
    • 序列相似性Belongs to the protein kinase superfamily. Tyr protein kinase family. FAK subfamily.
      Contains 1 FERM domain.
      Contains 1 protein kinase domain.
    • 结构域The first Pro-rich domain interacts with the SH3 domain of CRK-associated substrate (BCAR1) and CASL.
      The carboxy-terminal region is the site of focal adhesion targeting (FAT) sequence which mediates the localization of FAK1 to focal adhesions.
    • 翻译后修饰Phosphorylated on 6 tyrosine residues upon activation. Microtubule-induced dephosphorylation at Tyr-397 could be catalyzed by PTPN11 and regulated by ZFYVE21. Dephosphorylated by PTPN11 upon EPHA2 activation by its ligand EFNA1.
    • 细胞定位Cell junction > focal adhesion. Cell membrane. Constituent of focal adhesions.
    • Information by UniProt
    • 数据库链接
    • 别名
      • FADK 1 antibody
      • FADK antibody
      • FAK related non kinase polypeptide antibody
      • FAK1 antibody
      • FAK1_HUMAN antibody
      • Focal adhesion kinase 1 antibody
      • Focal adhesion Kinase antibody
      • Focal adhesion kinase isoform FAK Del33 antibody
      • Focal adhesion kinase related nonkinase antibody
      • FRNK antibody
      • p125FAK antibody
      • pp125FAK antibody
      • PPP1R71 antibody
      • Protein phosphatase 1 regulatory subunit 71 antibody
      • Protein tyrosine kinase 2 antibody
      • Protein-tyrosine kinase 2 antibody
      • Ptk2 antibody
      • PTK2 protein tyrosine kinase 2 antibody
      see all

    Anti-FAK (phospho Y576 + Y577) antibody [EP1832Y] 图像

    • All lanes : Anti-FAK (phospho Y576 + Y577) antibody [EP1832Y] (ab76244) at 1/50000 dilution

      Lane 1 : NIH-3T3 cell lysates, untreated
      Lane 2 : NIH-3T3 cell lysates treated with pervanadate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/1000 dilution

      Predicted band size : 119 kDa
      Observed band size : 125 kDa (why is the actual band size different from the predicted?)

    Anti-FAK (phospho Y576 + Y577) antibody [EP1832Y] (ab76244)参考文献

    This product has been referenced in:
    • Goodwin JM  et al. An AMPK-independent signaling pathway downstream of the LKB1 tumor suppressor controls Snail1 and metastatic potential. Mol Cell 55:436-50 (2014). Read more (PubMed: 25042806) »
    • Ratke J  et al. Leptin stimulates the migration of colon carcinoma cells by multiple signaling pathways. Endocr Relat Cancer 17:179-89 (2010). Read more (PubMed: 19952122) »

    See all 2 Publications for this product

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