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RabMAb

Anti-FAK (phospho Y576 + Y577)抗体[EP1832Y] (ab76244)

概述

  • 产品名称
    Anti-FAK (phospho Y576 + Y577)抗体[EP1832Y]
    参阅全部 FAK 一抗
  • 描述
    兔单克隆抗体[EP1832Y] to FAK (phospho Y576 + Y577)
  • 特异性
    Detects FAK phosphorylated at Y576/577.
  • 经测试应用
    适用于: WB, IP, ICC, Flow Cytmore details
    不适用于: IHC-P
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    A phospho specific peptide corresponding to residues surrounding Tyrosine 576/577 of human FAK.

  • 阳性对照
    • NIH-3T3 lysate treated with pervanadate.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

     

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

性能

应用

Our Abpromise guarantee covers the use of ab76244 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/50000 - 1/100000. Predicted molecular weight: 119 kDa.
IP 1/40.
ICC 1/50 - 1/100.
Flow Cyt 1/20.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • 应用说明
    Is unsuitable for IHC-P.
  • 靶标

    • 功能
      Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Microtubule-induced dephosphorylation at Tyr-397 is crucial for the induction of focal adhesion disassembly. Plays a potential role in oncogenic transformations resulting in increased kinase activity.
    • 组织特异性
      Expressed in all organs tested, in lymphoid cell lines, but most abundantly in brain.
    • 序列相似性
      Belongs to the protein kinase superfamily. Tyr protein kinase family. FAK subfamily.
      Contains 1 FERM domain.
      Contains 1 protein kinase domain.
    • 结构域
      The first Pro-rich domain interacts with the SH3 domain of CRK-associated substrate (BCAR1) and CASL.
      The carboxy-terminal region is the site of focal adhesion targeting (FAT) sequence which mediates the localization of FAK1 to focal adhesions.
    • 翻译后修饰
      Phosphorylated on 6 tyrosine residues upon activation. Microtubule-induced dephosphorylation at Tyr-397 could be catalyzed by PTPN11 and regulated by ZFYVE21. Dephosphorylated by PTPN11 upon EPHA2 activation by its ligand EFNA1.
    • 细胞定位
      Cell junction > focal adhesion. Cell membrane. Constituent of focal adhesions.
    • Information by UniProt
    • 数据库链接
    • 别名
      • FADK 1 antibody
      • FADK antibody
      • FAK related non kinase polypeptide antibody
      • FAK1 antibody
      • FAK1_HUMAN antibody
      • Focal adhesion kinase 1 antibody
      • Focal adhesion Kinase antibody
      • Focal adhesion kinase isoform FAK Del33 antibody
      • Focal adhesion kinase related nonkinase antibody
      • FRNK antibody
      • p125FAK antibody
      • pp125FAK antibody
      • PPP1R71 antibody
      • Protein phosphatase 1 regulatory subunit 71 antibody
      • Protein tyrosine kinase 2 antibody
      • Protein-tyrosine kinase 2 antibody
      • Ptk2 antibody
      • PTK2 protein tyrosine kinase 2 antibody
      see all

    图片

    • Anti-FAK (phospho Y576 + Y577) antibody [EP1832Y] (ab76244) Dot Blot. Primary ab dilution 1:1000,  Secondary ab description and code (ab id), Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051), Secondary ab dilution 1:100,000, Blocking buffer and concentration 5% NFDM/TBST, Diluting buffer and concentration 5% NFDM /TBST, Lane 1:FAK (pY576+pY577) phospho peptide, Lane 2:FAK (pY576) phospho peptide, Lane 3:FAK (pY577) phospho peptide, Lane 4:FAK non-phospho peptide, Exposure time 3 minutes. Note: antibody used for ab76244 is purified batch.

    • All lanes : Anti-FAK (phospho Y576 + Y577) antibody [EP1832Y] (ab76244) at 1/50000 dilution

      Lane 1 : NIH-3T3 cell lysates, untreated
      Lane 2 : NIH-3T3 cell lysates treated with pervanadate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/1000 dilution

      Predicted band size : 119 kDa
      Observed band size : 125 kDa (why is the actual band size different from the predicted?)

    文献

    This product has been referenced in:
    • Bernusso VA  et al. Imatinib restores VASP activity and its interaction with Zyxin in BCR-ABL leukemic cells. Biochim Biophys Acta 1853:388-95 (2015). Read more (PubMed: 25450971) »
    • Goodwin JM  et al. An AMPK-independent signaling pathway downstream of the LKB1 tumor suppressor controls Snail1 and metastatic potential. Mol Cell 55:436-50 (2014). Read more (PubMed: 25042806) »

    See all 4 Publications for this product

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