RabMAb

Anti-FAK (phospho Y576)抗体[EP1897Y] (ab76120)

概述

  • 产品名称Anti-FAK (phospho Y576)抗体[EP1897Y]
    参阅全部 FAK 一抗
  • 描述
    兔单克隆抗体[EP1897Y] to FAK (phospho Y576)
  • 特异性ab76120 detects FAK phosphorylated at Y576.
  • 经测试应用适用于: WB, Flow Cytmore details
    不适用于: ICC,IHC-P or IP
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    A phospho-specific peptide corresponding to residues surrounding Tyrosine 576 of human FAK

  • 阳性对照
    • 3T3 cell lysates treated with pervanadate.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

应用

Our Abpromise guarantee covers the use of ab76120 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/50000 - 1/100000. Detects a band of approximately 125 kDa (predicted molecular weight: 119 kDa).
Flow Cyt 1/50.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • 应用说明Is unsuitable for ICC,IHC-P or IP.
  • 靶标

    • 功能Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Microtubule-induced dephosphorylation at Tyr-397 is crucial for the induction of focal adhesion disassembly. Plays a potential role in oncogenic transformations resulting in increased kinase activity.
    • 组织特异性Expressed in all organs tested, in lymphoid cell lines, but most abundantly in brain.
    • 序列相似性Belongs to the protein kinase superfamily. Tyr protein kinase family. FAK subfamily.
      Contains 1 FERM domain.
      Contains 1 protein kinase domain.
    • 结构域The first Pro-rich domain interacts with the SH3 domain of CRK-associated substrate (BCAR1) and CASL.
      The carboxy-terminal region is the site of focal adhesion targeting (FAT) sequence which mediates the localization of FAK1 to focal adhesions.
    • 翻译后修饰Phosphorylated on 6 tyrosine residues upon activation. Microtubule-induced dephosphorylation at Tyr-397 could be catalyzed by PTPN11 and regulated by ZFYVE21. Dephosphorylated by PTPN11 upon EPHA2 activation by its ligand EFNA1.
    • 细胞定位Cell junction > focal adhesion. Cell membrane. Constituent of focal adhesions.
    • Information by UniProt
    • 数据库链接
    • 别名
      • FADK 1 antibody
      • FADK antibody
      • FAK related non kinase polypeptide antibody
      • FAK1 antibody
      • FAK1_HUMAN antibody
      • Focal adhesion kinase 1 antibody
      • Focal adhesion Kinase antibody
      • Focal adhesion kinase isoform FAK Del33 antibody
      • Focal adhesion kinase related nonkinase antibody
      • FRNK antibody
      • p125FAK antibody
      • pp125FAK antibody
      • PPP1R71 antibody
      • Protein phosphatase 1 regulatory subunit 71 antibody
      • Protein tyrosine kinase 2 antibody
      • Protein-tyrosine kinase 2 antibody
      • Ptk2 antibody
      • PTK2 protein tyrosine kinase 2 antibody
      see all

    Anti-FAK (phospho Y576) antibody [EP1897Y] 图像

    • All lanes : Anti-FAK (phospho Y576) antibody [EP1897Y] (ab76120) at 1/50000 dilution

      Lane 1 : 3T3 cell lysates untreated
      Lane 2 : 3T3 cell lysates treated with pervanadate.

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 119 kDa
      Observed band size : 125 kDa (why is the actual band size different from the predicted?)

    Anti-FAK (phospho Y576) antibody [EP1897Y] (ab76120)参考文献

    This product has been referenced in:
    • Strell C  et al. Neutrophil granulocytes promote the migratory activity of MDA-MB-468 human breast carcinoma cells via ICAM-1. Exp Cell Res 316:138-48 (2010). Read more (PubMed: 19747913) »

    See 1 Publication for this product

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    Thank you for contacting us.

    I heard back from the lab explaining that they used permeabilized Jurkat cells, butdo not use mouse samples for flow cytometry. The flow cytometry image is attached.

    Nevertheless, we guarantee the antib...

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    Thank you for contacting us.

    I just emailed the lab regarding the mouse samples tested in flow cytometry.

    The protocol is as follows:

    Flow Cytometry Protocol for Intracellular Staining

    1. Solutions and Reagent...

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    Thank you for contacting us.

    Please find attached the protocol we recomend for ttis antibody.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"