概述

  • 产品名称
  • 描述
    兔多克隆抗体to FAK
  • 经测试应用
    适用于: WB, IPmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    His-FAK, recombinant fragment, corresponding to amino acids 706-1052 of Human FAK, purified from E.coli.

  • 阳性对照
    • SK-N-MC and A431 cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab71563 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IP
  • 应用说明
    IP: Use 2µl/400µl lysate.
    WB: 1/1000. Detects bands of approximately 70 and 119 kDa (predicted molecular weight: 119 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能
      Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Microtubule-induced dephosphorylation at Tyr-397 is crucial for the induction of focal adhesion disassembly. Plays a potential role in oncogenic transformations resulting in increased kinase activity.
    • 组织特异性
      Expressed in all organs tested, in lymphoid cell lines, but most abundantly in brain.
    • 序列相似性
      Belongs to the protein kinase superfamily. Tyr protein kinase family. FAK subfamily.
      Contains 1 FERM domain.
      Contains 1 protein kinase domain.
    • 结构域
      The first Pro-rich domain interacts with the SH3 domain of CRK-associated substrate (BCAR1) and CASL.
      The carboxy-terminal region is the site of focal adhesion targeting (FAT) sequence which mediates the localization of FAK1 to focal adhesions.
    • 翻译后修饰
      Phosphorylated on 6 tyrosine residues upon activation. Microtubule-induced dephosphorylation at Tyr-397 could be catalyzed by PTPN11 and regulated by ZFYVE21. Dephosphorylated by PTPN11 upon EPHA2 activation by its ligand EFNA1.
    • 细胞定位
      Cell junction > focal adhesion. Cell membrane. Constituent of focal adhesions.
    • Information by UniProt
    • 数据库链接
    • 别名
      • FADK 1 antibody
      • FADK antibody
      • FAK related non kinase polypeptide antibody
      • FAK1 antibody
      • FAK1_HUMAN antibody
      • Focal adhesion kinase 1 antibody
      • Focal adhesion Kinase antibody
      • Focal adhesion kinase isoform FAK Del33 antibody
      • Focal adhesion kinase related nonkinase antibody
      • FRNK antibody
      • p125FAK antibody
      • pp125FAK antibody
      • PPP1R71 antibody
      • Protein phosphatase 1 regulatory subunit 71 antibody
      • Protein tyrosine kinase 2 antibody
      • Protein-tyrosine kinase 2 antibody
      • Ptk2 antibody
      • PTK2 protein tyrosine kinase 2 antibody
      see all

    图片

    • All lanes : Anti-FAK antibody (ab71563) at 1/1000 dilution

      Lane 1 : SK-N-MC cell lysate
      Lane 2 : A431 cell lysate


      Predicted band size : 119 kDa
      Observed band size : 119 kDa
      Additional bands at : 70 kDa. We are unsure as to the identity of these extra bands.

    文献

    ab71563 has not yet been referenced specifically in any publications.

    客户评价及客户问答

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunoprecipitation
    Sample
    Rat Cell lysate - whole cell (Oligodendrocyte)
    Total protein in input
    50 µg
    Specification
    Oligodendrocyte
    Immuno-precipitation step
    Protein A/G
    Username

    Abcam user community

    Verified customer

    提交于 Jan 11 2013

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Rat Cell lysate - whole cell (Oligodedroyte)
    Loading amount
    30 µg
    Specification
    Oligodedroyte
    Gel Running Conditions
    Reduced Denaturing (12)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    提交于 Jan 11 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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