概述

  • 产品名称Anti-FAK抗体
    参阅全部 FAK 一抗
  • 描述
    兔多克隆抗体to FAK
  • 经测试应用适用于: ELISA, IHC-P, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic non-phosphopeptide derived from Human FAK around the phosphorylation site of tyrosine 576 (S-T-Y-Y-K).

  • 阳性对照
    • Human brain tissue and extracts from NIH/3T3 cells.

性能

应用

Our Abpromise guarantee covers the use of ab61113 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/5000.
IHC-P 1/50 - 1/100.
WB 1/500 - 1/1000. Detects a band of approximately 119 kDa (predicted molecular weight: 119 kDa).

靶标

  • 功能Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Microtubule-induced dephosphorylation at Tyr-397 is crucial for the induction of focal adhesion disassembly. Plays a potential role in oncogenic transformations resulting in increased kinase activity.
  • 组织特异性Expressed in all organs tested, in lymphoid cell lines, but most abundantly in brain.
  • 序列相似性Belongs to the protein kinase superfamily. Tyr protein kinase family. FAK subfamily.
    Contains 1 FERM domain.
    Contains 1 protein kinase domain.
  • 结构域The first Pro-rich domain interacts with the SH3 domain of CRK-associated substrate (BCAR1) and CASL.
    The carboxy-terminal region is the site of focal adhesion targeting (FAT) sequence which mediates the localization of FAK1 to focal adhesions.
  • 翻译后修饰Phosphorylated on 6 tyrosine residues upon activation. Microtubule-induced dephosphorylation at Tyr-397 could be catalyzed by PTPN11 and regulated by ZFYVE21. Dephosphorylated by PTPN11 upon EPHA2 activation by its ligand EFNA1.
  • 细胞定位Cell junction > focal adhesion. Cell membrane. Constituent of focal adhesions.
  • Information by UniProt
  • 数据库链接
  • 别名
    • FADK 1 antibody
    • FADK antibody
    • FAK related non kinase polypeptide antibody
    • FAK1 antibody
    • FAK1_HUMAN antibody
    • Focal adhesion kinase 1 antibody
    • Focal adhesion Kinase antibody
    • Focal adhesion kinase isoform FAK Del33 antibody
    • Focal adhesion kinase related nonkinase antibody
    • FRNK antibody
    • p125FAK antibody
    • pp125FAK antibody
    • PPP1R71 antibody
    • Protein phosphatase 1 regulatory subunit 71 antibody
    • Protein tyrosine kinase 2 antibody
    • Protein-tyrosine kinase 2 antibody
    • Ptk2 antibody
    • PTK2 protein tyrosine kinase 2 antibody
    see all

Anti-FAK antibody 图像

  • ab61113 at a 1/50 dilution, staining human FAK in brain tissue with (+) or without (-) immunising peptide, using Immunohistochemistry, Paraffin embedded tissue
  • All lanes : Anti-FAK antibody (ab61113) at 1/500 dilution

    Lane 1 : Extracts from NIH/3T3 cells
    Lane 2 : Extracts from NIH/3T3 cells with immunising FAK peptide


    Predicted band size : 119 kDa
    Observed band size : 119 kDa
    Additional bands at : 70 kDa. We are unsure as to the identity of these extra bands.

Anti-FAK antibody (ab61113)参考文献

This product has been referenced in:
  • Oulidi A  et al. TRPV2 mediates adrenomedullin stimulation of prostate and urothelial cancer cell adhesion, migration and invasion. PLoS One 8:e64885 (2013). WB ; Human . Read more (PubMed: 23741410) »
  • Vandenberghe M  et al. ORAI1 calcium channel orchestrates skin homeostasis. Proc Natl Acad Sci U S A 110:E4839-48 (2013). WB ; Human . Read more (PubMed: 24277812) »

See all 3 Publications for this product

Product Wall

Application Western blot
Sample Pig Cell lysate - whole cell (Porcine primary aortic endothelial cells)
Gel Running Conditions Reduced Denaturing (12% Bis-Tris gel)
Loading amount 3.8 µg
Specification Porcine primary aortic endothelial cells
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
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Verified customer

提交于 Jul 03 2015

Thank you very much for your interest in our antibodies.

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Thank you for your reply.
Reviewing the remaining antibodies that are already tested in WB and the species you wish to use, the following do not yet have a western blot image, and I would be pleased to arrange a testing discount for these addition...

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