概述

  • 产品名称
  • 描述
    兔多克隆抗体to FADS2
  • 经测试应用
    适用于: WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Monkey, Baboon, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human FADS2.

    (Peptide available as ab90992.)

  • 阳性对照
    • This antibody gave a positive signal in the following lysates: Human liver tissue lysate; Human brain tissue lysates; HeLa whole cell lysate; Hep G2 whole cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab72189 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 52 kDa).
IP Use at an assay dependent concentration.

靶标

  • 功能
    Component of a lipid metabolic pathway that catalyzes biosynthesis of highly unsaturated fatty acids (HUFA) from precursor essential polyunsaturated fatty acids (PUFA) linoleic acid (LA) (18:2n-6) and alpha-linolenic acid (ALA) (18:3n-3). Catalyzes the first and rate limiting step in this pathway which is the desaturation of LA (18:2n-6) and ALA (18:3n-3) into gamma-linoleic acid (GLA) (18:3n-6) and stearidonic acid (18:4n-3) respectively and other desaturation steps. Highly unsaturated fatty acids (HUFA) play pivotal roles in many biological functions. It catalizes as well the introduction of a cis double bond in palmitate to produce the mono-unsaturated fatty acid sapienate, the most abundant fatty acid in sebum.
  • 组织特异性
    Expressed in a wide array of tissues, highest expression is found in liver followed by brain, lung, heart, and retina. A lower level is found in breast tumor when compared with normal tissues; lowest levels were found in patients with poor prognostic index.
  • 通路
    Lipid metabolism; polyunsaturated fatty acid biosynthesis.
  • 序列相似性
    Belongs to the fatty acid desaturase family.
    Contains 1 cytochrome b5 heme-binding domain.
  • 发展阶段
    Found in fetal heart.
  • 结构域
    The histidine box domains may contain the active site and/or be involved in metal ion binding.
  • 细胞定位
    Endoplasmic reticulum membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • D6D antibody
    • Delta(6) desaturase antibody
    • Delta(6) fatty acid desaturase antibody
    • Delta-6 desaturase antibody
    • DES 6 antibody
    • DES6 antibody
    • FADS 2 antibody
    • FADS2 antibody
    • FADS2_HUMAN antibody
    • FADSD 6 antibody
    • FADSD6 antibody
    • Fatty acid desaturase 2 antibody
    • linoleoyl-CoA desaturase (delta-6-desaturase) like 2 antibody
    • LLCDL 2 antibody
    • LLCDL2 antibody
    • SLL0262 antibody
    • TU 13 antibody
    • TU13 antibody
    see all

图片

  • FADS2 was immunoprecipitated using 0.5mg HepG2 whole cell extract, 5µg of Rabbit polyclonal to FADS2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, HepG2 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab72189.
    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
    Band: 45kDa:FADS2.
  • All lanes : Anti-FADS2 antibody (ab72189) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Human liver tissue lysate - total protein (ab29889)
    Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 4 : Human brain tissue lysate - total protein (ab29466)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 52 kDa
    Observed band size : 45 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 62.5 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 2 minutesab72189 detects a strong band at 45 kDa in human liver and brain tissue lysates, aswell as hela and hepG2 whole cell lysates. This antibody was raised against an immunogen that is predicted to cross react with all known isoforms of Fatty acid desaturase 2 (FADS2). FADS2 has three isoforms, isoform one has a molecular weight of 52 kDa with isoforms two and three a molecular weight of 49 and 45 respectively(SwissProt data). The band we have observed is thought to represent isoform 3 of FADS2 (45 kDa).

文献

This product has been referenced in:
  • Naudí A  et al. Region-specific vulnerability to lipid peroxidation and evidence of neuronal mechanisms for polyunsaturated fatty acid biosynthesis in the healthy adult human central nervous system. Biochim Biophys Acta 1862:485-495 (2017). WB ; Human . Read more (PubMed: 28185952) »

See 1 Publication for this product

客户评价及客户问答



We have in our catalog one anti-FADS2, the reference ishttp://www.abcam.com/ab72189.Unfortunately the homology between the immunogen peptide and therainbow trout FADS2 is only 27% (see attachment). It is therefore veryunlikely this antibody i...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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