Anti-FADD (phospho S194)抗体(ab55334)

概述

  • 产品名称Anti-FADD (phospho S194)抗体
    参阅全部 FADD 一抗
  • 描述
    兔多克隆抗体to FADD (phospho S194)
  • 特异性This antibody detects endogenous levels of FADD only when phosphorylated at serine 194.
  • 经测试应用适用于: ELISA, WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Synthetic phosphopeptide derived from human FADD around the phosphorylation site of serine 194 (A-M-SP-P-M).

  • 阳性对照
    • Extracts from HeLa cells.

性能

应用

Our Abpromise guarantee covers the use of ab55334 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/1000.
WB 1/500 - 1/1000. Detects a band of approximately 30 kDa (predicted molecular weight: 23 kDa).
IHC-P 1/50 - 1/100.

靶标

  • 功能Apoptotic adaptor molecule that recruits caspase-8 or caspase-10 to the activated Fas (CD95) or TNFR-1 receptors. The resulting aggregate called the death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation. Active caspase-8 initiates the subsequent cascade of caspases mediating apoptosis.
  • 组织特异性Expressed in a wide variety of tissues, except for peripheral blood mononuclear leukocytes.
  • 序列相似性Contains 1 death domain.
    Contains 1 DED (death effector) domain.
  • 结构域Contains a death domain involved in the binding of the corresponding domain within Fas receptor.
  • Information by UniProt
  • 数据库链接
  • 别名
    • FADD antibody
    • FADD protein antibody
    • FADD_HUMAN antibody
    • Fas (TNFRSF6) associated via death domain antibody
    • Fas associated via death domain antibody
    • Fas associating death domain containing protein antibody
    • Fas associating protein antibody
    • Fas associating protein with death domain antibody
    • Fas TNFRSF6 associated via death domain antibody
    • FAS-associated death domain protein antibody
    • FAS-associating death domain-containing protein antibody
    • GIG 3 antibody
    • GIG3 antibody
    • Growth inhibiting gene 3 protein antibody
    • Growth-inhibiting gene 3 protein antibody
    • H sapiens mRNA for mediator of receptor induced toxicity antibody
    • Mediator of receptor induced toxicity antibody
    • MGC8528 antibody
    • MORT 1 antibody
    • MORT1 antibody
    • Protein FADD antibody
    see all

Anti-FADD (phospho S194) antibody 图像

  • All lanes : Anti-FADD (phospho S194) antibody (ab55334) at 1/500 dilution

    Lane 1 : Extracts from HeLa cells treated with Paclitaxel (1µM, 60mins).
    Lane 2 : Extracts from HeLa cells treated with Paclitaxel (1µM, 60mins), and with the immunising phosphpeptide.


    Predicted band size : 23 kDa
    Observed band size : 30 kDa (why is the actual band size different from the predicted?)

Anti-FADD (phospho S194) antibody (ab55334)参考文献

ab55334 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Rabbit Cell lysate - whole cell (Rabbit Reticulocyte)
Gel Running Conditions Reduced Denaturing (4-12% Bolt Bis-Tris Plus gel (MES buffer))
Loading amount 0.5 µg
Specification Rabbit Reticulocyte
Blocking step Li-Cor Odyssey Blocking Buffer (TBS) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
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提交于 Dec 01 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Hamster Cell lysate - whole cell (BHK-21 (Kidney Fibroblasts))
Gel Running Conditions Reduced Denaturing (4-12% Bolt Bis-Tris Plus gel)
Loading amount 500000 cells
Specification BHK-21 (Kidney Fibroblasts)
Blocking step Li-Cor Odyssey Blocking Buffer (TBS) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
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提交于 Oct 31 2016

Application Western blot
Sample Dog Cell lysate - whole cell (Canine Kidney Epithelial MDCK cells)
Gel Running Conditions Reduced Denaturing (4-12% Bolt Bis-Tris Plus gel)
Loading amount 500000 cells
Specification Canine Kidney Epithelial MDCK cells
Blocking step Li-Cor Odyssey Blocking Buffer (TBS) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
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提交于 Oct 21 2016

Application Western blot
Sample Human Cell lysate - whole cell (Human Foreskin Fibroblasts)
Gel Running Conditions Reduced Denaturing (4-12% Bolt Bis-Tris Plus gradient gel)
Loading amount 200000 cells
Specification Human Foreskin Fibroblasts
Blocking step Li-Cor Odyssey Blocking Buffer (TBS) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
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提交于 Feb 09 2016

Application Western blot
Sample Mouse Cell lysate - whole cell (mouse embryonic fibroblasts (MEFs))
Gel Running Conditions Reduced Denaturing (4-12% Bolt Bis-Tris Plus gradient gel)
Loading amount 200000 cells
Specification mouse embryonic fibroblasts (MEFs)
Blocking step Li-Cor Odyssey Blocking Buffer (TBS) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
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提交于 Feb 09 2016

I am very pleased to hear you would like to accept our offer and test ab55334 on mouse samples by western blotting. This code will give you one free primary antibody before the expiration date. To redeem this offer, please submit an Abreview of mouse r...

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