Anti-FADD (phospho S191)抗体(ab111379)

概述

  • 产品名称
    Anti-FADD (phospho S191)抗体
    参阅全部 FADD 一抗
  • 描述
    兔多克隆抗体to FADD (phospho S191)
  • 特异性
    ab111379 detects endogenous levels of FADD only when phosphorylated at serine 191.
  • 经测试应用
    适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Synthetic peptide corresponding to Mouse FADD (C terminal) (phospho S191).
    Sequence:

    NMSPV

  • 阳性对照
    • Human breast carcinoma tissue; Jurkat cell extracts, treated with PMA (125ng/ml, 30mins).

性能

应用

Our Abpromise guarantee covers the use of ab111379 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Predicted molecular weight: 23 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能
    Apoptotic adaptor molecule that recruits caspase-8 or caspase-10 to the activated Fas (CD95) or TNFR-1 receptors. The resulting aggregate called the death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation. Active caspase-8 initiates the subsequent cascade of caspases mediating apoptosis.
  • 组织特异性
    Expressed in a wide variety of tissues, except for peripheral blood mononuclear leukocytes.
  • 序列相似性
    Contains 1 death domain.
    Contains 1 DED (death effector) domain.
  • 结构域
    Contains a death domain involved in the binding of the corresponding domain within Fas receptor.
  • Information by UniProt
  • 数据库链接
  • 别名
    • FADD antibody
    • FADD protein antibody
    • FADD_HUMAN antibody
    • Fas (TNFRSF6) associated via death domain antibody
    • Fas associated via death domain antibody
    • Fas associating death domain containing protein antibody
    • Fas associating protein antibody
    • Fas associating protein with death domain antibody
    • Fas TNFRSF6 associated via death domain antibody
    • FAS-associated death domain protein antibody
    • FAS-associating death domain-containing protein antibody
    • GIG 3 antibody
    • GIG3 antibody
    • Growth inhibiting gene 3 protein antibody
    • Growth-inhibiting gene 3 protein antibody
    • H sapiens mRNA for mediator of receptor induced toxicity antibody
    • Mediator of receptor induced toxicity antibody
    • MGC8528 antibody
    • MORT 1 antibody
    • MORT1 antibody
    • Protein FADD antibody
    see all

图片

  • All lanes : Anti-FADD (phospho S191) antibody (ab111379) at 1/500 dilution

    Lane 1 : Jurkat cell extracts, treated with PMA (125ng/ml, 30mins)
    Lane 2 : Jurkat cell extracts, treated with PMA (125ng/ml, 30mins) with immunizing peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 23 kDa
  • ab111379 at 1/50 dilution staining FADD in paraffin-embedded Human Breast carcinoma tissue by Immunohistochemistry. The image on the right is treated with the synthesized phosphopeptide.

文献

ab111379 has not yet been referenced specifically in any publications.

客户评价及客户问答

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Chinese hamster Cell lysate - whole cell (Chinese hamster ovary (CHO) cells)
Gel Running Conditions
Reduced Denaturing (4-12% Bolt Bis-Tris Plus gel (MOPS buffer))
Loading amount
50000 cells
Specification
Chinese hamster ovary (CHO) cells
Blocking step
Li-Cor Odyssey Blocking Buffer (TBS) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Jan 17 2017

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Dog Cell lysate - whole cell (Canine Kidney Epithelial MDCK cells)
Gel Running Conditions
Reduced Denaturing (4-12% Bolt Bis-Tris Plus gel (MOPS buffer))
Loading amount
25000 cells
Specification
Canine Kidney Epithelial MDCK cells
Blocking step
Li-Cor Odyssey Blocking Buffer (TBS) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Jan 17 2017

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
African green monkey Cell lysate - whole cell (VERO E6)
Gel Running Conditions
Reduced Denaturing (4-12% Bolt Bis-Tris Plus gel (MOPS buffer))
Loading amount
75000 cells
Specification
VERO E6
Blocking step
Li-Cor Odyssey Blocking Buffer (TBS) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Jan 17 2017

Application
Western blot
Sample
Human Cell lysate - whole cell (Human Foreskin Fibroblasts)
Gel Running Conditions
Reduced Denaturing (4-12% Bolt Bis-Tris Plus gradient gel)
Loading amount
200000 cells
Specification
Human Foreskin Fibroblasts
Blocking step
Li-Cor Odyssey Blocking Buffer (TBS) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Feb 19 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (mouse embryonic fibroblasts (MEFs))
Gel Running Conditions
Reduced Denaturing (4-12% Bolt Bis-Tris Plus gradient gel)
Loading amount
200000 cells
Specification
mouse embryonic fibroblasts (MEFs)
Blocking step
Li-Cor Odyssey Blocking Buffer (TBS) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Feb 09 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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