Anti-FADD (phospho S191)抗体(ab111379)

概述

  • 产品名称Anti-FADD (phospho S191)抗体
    参阅全部 FADD 一抗
  • 描述
    兔多克隆抗体to FADD (phospho S191)
  • 特异性ab111379 detects endogenous levels of FADD only when phosphorylated at serine 191.
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Synthetic peptide corresponding to Mouse FADD (C terminal) (phospho S191).
    Sequence:

    NMSPV

  • 阳性对照
    • Human breast carcinoma tissue; Jurkat cell extracts, treated with PMA (125ng/ml, 30mins).

性能

应用

Our Abpromise guarantee covers the use of ab111379 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Predicted molecular weight: 23 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能Apoptotic adaptor molecule that recruits caspase-8 or caspase-10 to the activated Fas (CD95) or TNFR-1 receptors. The resulting aggregate called the death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation. Active caspase-8 initiates the subsequent cascade of caspases mediating apoptosis.
  • 组织特异性Expressed in a wide variety of tissues, except for peripheral blood mononuclear leukocytes.
  • 序列相似性Contains 1 death domain.
    Contains 1 DED (death effector) domain.
  • 结构域Contains a death domain involved in the binding of the corresponding domain within Fas receptor.
  • Information by UniProt
  • 数据库链接
  • 别名
    • FADD antibody
    • FADD protein antibody
    • FADD_HUMAN antibody
    • Fas (TNFRSF6) associated via death domain antibody
    • Fas associated via death domain antibody
    • Fas associating death domain containing protein antibody
    • Fas associating protein antibody
    • Fas associating protein with death domain antibody
    • Fas TNFRSF6 associated via death domain antibody
    • FAS-associated death domain protein antibody
    • FAS-associating death domain-containing protein antibody
    • GIG 3 antibody
    • GIG3 antibody
    • Growth inhibiting gene 3 protein antibody
    • Growth-inhibiting gene 3 protein antibody
    • H sapiens mRNA for mediator of receptor induced toxicity antibody
    • Mediator of receptor induced toxicity antibody
    • MGC8528 antibody
    • MORT 1 antibody
    • MORT1 antibody
    • Protein FADD antibody
    see all

Anti-FADD (phospho S191) antibody 图像

  • All lanes : Anti-FADD (phospho S191) antibody (ab111379) at 1/500 dilution

    Lane 1 : Jurkat cell extracts, treated with PMA (125ng/ml, 30mins)
    Lane 2 : Jurkat cell extracts, treated with PMA (125ng/ml, 30mins) with immunizing peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 23 kDa
  • ab111379 at 1/50 dilution staining FADD in paraffin-embedded Human Breast carcinoma tissue by Immunohistochemistry. The image on the right is treated with the synthesized phosphopeptide.

Anti-FADD (phospho S191) antibody (ab111379)参考文献

ab111379 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (Human Foreskin Fibroblasts)
Gel Running Conditions Reduced Denaturing (4-12% Bolt Bis-Tris Plus gradient gel)
Loading amount 200000 cells
Specification Human Foreskin Fibroblasts
Blocking step Li-Cor Odyssey Blocking Buffer (TBS) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
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提交于 Feb 19 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (mouse embryonic fibroblasts (MEFs))
Gel Running Conditions Reduced Denaturing (4-12% Bolt Bis-Tris Plus gradient gel)
Loading amount 200000 cells
Specification mouse embryonic fibroblasts (MEFs)
Blocking step Li-Cor Odyssey Blocking Buffer (TBS) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Feb 09 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"