The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/10000. Detects a band of approximately 28 kDa (predicted molecular weight: 23 kDa).
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
Use at an assay dependent concentration.
应用说明Is unsuitable for ICC or IP.
功能Apoptotic adaptor molecule that recruits caspase-8 or caspase-10 to the activated Fas (CD95) or TNFR-1 receptors. The resulting aggregate called the death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation. Active caspase-8 initiates the subsequent cascade of caspases mediating apoptosis.
组织特异性Expressed in a wide variety of tissues, except for peripheral blood mononuclear leukocytes.
序列相似性Contains 1 death domain. Contains 1 DED (death effector) domain.
结构域Contains a death domain involved in the binding of the corresponding domain within Fas receptor.