Anti-Factor XII heavy chain cleaved Arg372抗体(ab79554)

概述

  • 产品名称Anti-Factor XII heavy chain cleaved Arg372抗体
  • 描述
    兔多克隆抗体to Factor XII heavy chain cleaved Arg372
  • 特异性Detects the fragment of activated Factor XII resulting from cleavage adjacent to Arg372.
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide (Human)

  • 阳性对照
    • Extracts from 293 cells.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab79554 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 42 kDa.
ELISA 1/20000.

靶标

  • 功能Factor XII is a serum glycoprotein that participates in the initiation of blood coagulation, fibrinolysis, and the generation of bradykinin and angiotensin. Prekallikrein is cleaved by factor XII to form kallikrein, which then cleaves factor XII first to alpha-factor XIIa and then trypsin cleaves it to beta-factor XIIa. Alpha-factor XIIa activates factor XI to factor XIa.
  • 疾病相关Factor XII deficiency
    Hereditary angioedema 3
  • 序列相似性Belongs to the peptidase S1 family.
    Contains 2 EGF-like domains.
    Contains 1 fibronectin type-I domain.
    Contains 1 fibronectin type-II domain.
    Contains 1 kringle domain.
    Contains 1 peptidase S1 domain.
  • 翻译后修饰Factor XII is activated by kallikrein in alpha-factor XIIa, which is further converted by trypsin into beta-factor XIIa. Alpha-factor XIIa is composed of an NH2-terminal heavy chain, called coagulation factor XIIa heavy chain, and a COOH-terminal light chain, called coagulation factor XIIa light chain, connected by a disulfide bond. Beta-factor XIIa is composed of 2 chains linked by a disulfide bond, an N-terminal nonapeptide, called beta-factor XIIa part 1, and coagulation factor XIIa light chain, also known in this context as beta-factor XIIa part 2.
    O- and N-glycosylated. The O-linked polysaccharides were not identified, but are probably the mucin type linked to GalNAc.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Beta factor XIIa part 1 antibody
    • Beta factor XIIa part 2 antibody
    • Beta-factor XIIa part 2 antibody
    • Coagulation factor XII antibody
    • Coagulation factor XII (Hageman factor) antibody
    • Coagulation factor XIIa heavy chain antibody
    • Coagulation factor XIIa light chain antibody
    • F12 antibody
    • FA12 antibody
    • FA12_HUMAN antibody
    • HAE3 antibody
    • HAEX antibody
    • HAF antibody
    • Hageman factor antibody
    see all

Anti-Factor XII heavy chain cleaved Arg372 antibody 图像

  • All lanes : Anti-Factor XII heavy chain cleaved Arg372 antibody (ab79554) at 1/500 dilution

    Lane 1 : Extracts from 293 cells, treated with etoposide (25uM, 1hour)
    Lane 2 : Extracts from 293 cells, treated with etoposide (25uM, 1hour) with immunizing peptide at 5 µg

    Lysates/proteins at 25 µg per lane.


    Observed band size : 42 kDa (why is the actual band size different from the predicted?)

Anti-Factor XII heavy chain cleaved Arg372 antibody (ab79554)参考文献

ab79554 has not yet been referenced specifically in any publications.

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