Anti-Factor X抗体(ab79929)

概述

  • 产品名称Anti-Factor X抗体
    参阅全部 Factor X 一抗
  • 描述
    兔多克隆抗体to Factor X
  • 经测试应用适用于: ICC/IF, Flow Cyt, IHC-P, WB, RIAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Human Factor X purified from human plasma

  • 阳性对照
    • This antibody gave a positive result in IHC in the following FFPE tissue: Human normal liver. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: HepG2.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 50% Glycerol, PBS, pH 7.5
  • Concentration information loading...
  • 纯度Protein G purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab79929 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF
Flow Cyt
IHC-P
WB
RIA
  • 应用说明 EIA/RIA: Use at a concentration of 5 µg/ml.
    WB: Use at an assay dependent dilution. Predicted molecular weight: 55 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Factor Xa is a vitamin K-dependent glycoprotein that converts prothrombin to thrombin in the presence of factor Va, calcium and phospholipid during blood clotting.
    • 组织特异性Plasma; synthesized in the liver.
    • 疾病相关Defects in F10 are the cause of factor X deficiency (FA10D) [MIM:227600]. A hemorrhagic disease with variable presentation. Affected individuals can manifest prolonged nasal and mucosal hemorrhage, menorrhagia, hematuria, and occasionally hemarthrosis. Some patients do not have clinical bleeding diathesis.
    • 序列相似性Belongs to the peptidase S1 family.
      Contains 2 EGF-like domains.
      Contains 1 Gla (gamma-carboxy-glutamate) domain.
      Contains 1 peptidase S1 domain.
    • 翻译后修饰The vitamin K-dependent, enzymatic carboxylation of some glutamate residues allows the modified protein to bind calcium.
      N- and O-glycosylated.
      The activation peptide is cleaved by factor IXa (in the intrinsic pathway), or by factor VIIa (in the extrinsic pathway).
      The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.
    • 细胞定位Secreted.
    • Information by UniProt
    • 数据库链接
    • 形式Cleaved into the following 3 chains: 1. Factor X light chain 2. Factor X heavy chain 3. Activated factor Xa heavy chain
    • 别名
      • Activated factor Xa heavy chain antibody
      • Coagulation factor X antibody
      • F10 antibody
      • FA10_HUMAN antibody
      • factor Xa antibody
      • FX antibody
      • FXA antibody
      • Prothrombinase antibody
      • Stuart factor antibody
      • Stuart Prower factor antibody
      • Stuart-Prower factor antibody
      see all

    Anti-Factor X antibody 图像

    • ICC/IF image of ab79929 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab79929 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    • IHC image of Factor X staining in Human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab79929, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    Anti-Factor X antibody (ab79929)参考文献

    ab79929 has not yet been referenced specifically in any publications.

    Product Wall

    Application Flow Cytometry
    Fixation Formaldehyde
    Sample Human Cell (hepatocyte)
    Specification hepatocyte
    Permeabilization Yes - 0.1% triton
    Username

    Dr. Xaowen He

    Verified customer

    提交于 Nov 27 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"