Anti-Factor VIII抗体[2.2.9] (ab41186)

概述

  • 产品名称Anti-Factor VIII抗体[2.2.9]
    参阅全部 Factor VIII 一抗
  • 描述
    小鼠单克隆抗体[2.2.9] to Factor VIII
  • 特异性ab41186 specifically recognises full-length human Factor VIII.
  • 经测试应用适用于: IHC-P, WB, ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Purified human Factor VIII.

  • 阳性对照
    • This antibody gave a positive result in IHC in the following FFPE tissue: Human normal liver
  • 常规说明Stable for at least 1 year at -20°C to -70°C.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 纯度Protein G purified
  • 克隆单克隆
  • 克隆编号2.2.9
  • 同种型IgG1
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab41186 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use at an assay dependent concentration. Predicted molecular weight: 267 kDa.
ELISA 1/32000.

靶标

  • 功能Factor VIII, along with calcium and phospholipid, acts as a cofactor for factor IXa when it converts factor X to the activated form, factor Xa.
  • 疾病相关Defects in F8 are the cause of hemophilia A (HEMA) [MIM:306700]. A disorder of blood coagulation characterized by a permanent tendency to hemorrhage. About 50% of patients have severe hemophilia resulting in frequent spontaneous bleeding into joints, muscles and internal organs. Less severe forms are characterized by bleeding after trauma or surgery. Note=Of particular interest for the understanding of the function of F8 is the category of CRM (cross-reacting material) positive patients (approximately 5%) that have considerable amount of F8 in their plasma (at least 30% of normal), but the protein is non-functional; i.e., the F8 activity is much less than the plasma protein level. CRM-reduced is another category of patients in which the F8C antigen and activity are reduced to approximately the same level. Most mutations are CRM negative, and probably affect the folding and stability of the protein.
  • 序列相似性Belongs to the multicopper oxidase family.
    Contains 3 F5/8 type A domains.
    Contains 2 F5/8 type C domains.
    Contains 6 plastocyanin-like domains.
  • 结构域Domain F5/8 type C 2 is responsible for phospholipid-binding and essential for factor VIII activity.
  • 翻译后修饰Sulfation on Tyr-1699 is essential for binding vWF.
  • 细胞定位Secreted > extracellular space.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AHF antibody
    • Antihemophilic factor antibody
    • Coagulation factor VIII antibody
    • coagulation factor VIII, procoagulant component antibody
    • coagulation factor VIIIc antibody
    • DXS1253E antibody
    • F8 antibody
    • F8b antibody
    • F8c antibody
    • FA8_HUMAN antibody
    • factor VIII F8B antibody
    • Factor VIIIa light chain antibody
    • FactorVIII antibody
    • FVIII antibody
    • Hema antibody
    • Hemophilia A antibody
    • Hemophilia, classic antibody
    • OTTHUMP00000061446 antibody
    • OTTHUMP00000196174 antibody
    • Procoagulant component antibody
    see all

Anti-Factor VIII antibody [2.2.9] 图像

  • IHC image of Factor VIII staining in Human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab41186, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Factor VIII antibody [2.2.9] (ab41186)参考文献

ab41186 has not yet been referenced specifically in any publications.

Product Wall

You will receive this replacement vial tomorrow.

Sorry about that. I did set up the order for the correct replacement antibody, 1 vial of ab41186, but noted the wrong catalog number in the email to you.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab41188 with the order number 973153. To check the status of the orde...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"