Anti-Factor VIII抗体[2.2.32] (ab41187)

概述

  • 产品名称
    Anti-Factor VIII抗体[2.2.32]
    参阅全部 Factor VIII 一抗
  • 描述
    小鼠单克隆抗体[2.2.32] to Factor VIII
  • 特异性
    ab41187 recognises full-length human Factor VIII.
  • 经测试应用
    适用于: ICC/IF, WB, ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Purified human Factor VIII.

  • 阳性对照
    • This antibody gave a positive result when used in the following formaldehyde fixed cell lines: HeLa.
  • 常规说明

    Stable for at least 1 year at -20°C to -70°C.

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab139391 as a replacement.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液
    Preservative: None
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 纯度
    Protein G purified
  • 克隆
    单克隆
  • 克隆编号
    2.2.32
  • 同种型
    IgG1
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab41187 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
WB Use at an assay dependent dilution. Predicted molecular weight: 267 kDa.
ELISA 1/32000.

靶标

  • 功能
    Factor VIII, along with calcium and phospholipid, acts as a cofactor for factor IXa when it converts factor X to the activated form, factor Xa.
  • 疾病相关
    Defects in F8 are the cause of hemophilia A (HEMA) [MIM:306700]. A disorder of blood coagulation characterized by a permanent tendency to hemorrhage. About 50% of patients have severe hemophilia resulting in frequent spontaneous bleeding into joints, muscles and internal organs. Less severe forms are characterized by bleeding after trauma or surgery. Note=Of particular interest for the understanding of the function of F8 is the category of CRM (cross-reacting material) positive patients (approximately 5%) that have considerable amount of F8 in their plasma (at least 30% of normal), but the protein is non-functional; i.e., the F8 activity is much less than the plasma protein level. CRM-reduced is another category of patients in which the F8C antigen and activity are reduced to approximately the same level. Most mutations are CRM negative, and probably affect the folding and stability of the protein.
  • 序列相似性
    Belongs to the multicopper oxidase family.
    Contains 3 F5/8 type A domains.
    Contains 2 F5/8 type C domains.
    Contains 6 plastocyanin-like domains.
  • 结构域
    Domain F5/8 type C 2 is responsible for phospholipid-binding and essential for factor VIII activity.
  • 翻译后修饰
    Sulfation on Tyr-1699 is essential for binding vWF.
  • 细胞定位
    Secreted > extracellular space.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AHF antibody
    • Antihemophilic factor antibody
    • Coagulation factor VIII antibody
    • coagulation factor VIII, procoagulant component antibody
    • coagulation factor VIIIc antibody
    • DXS1253E antibody
    • F8 antibody
    • F8b antibody
    • F8c antibody
    • FA8_HUMAN antibody
    • factor VIII F8B antibody
    • Factor VIIIa light chain antibody
    • FactorVIII antibody
    • FVIII antibody
    • Hema antibody
    • Hemophilia A antibody
    • Hemophilia, classic antibody
    • OTTHUMP00000061446 antibody
    • OTTHUMP00000196174 antibody
    • Procoagulant component antibody
    see all

图片

  • ICC/IF image of ab41187 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab41187 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- mouse (ab96879) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

文献

ab41187 has not yet been referenced specifically in any publications.

客户评价及客户问答

Thank you for contacting us.

I’ve attached the ELISA protocol we use for testing our Factor VIII MAbs. We use native human Factor VIII purified from plasma –we have not tried recombinant human Factor VIII.

I hope this ...

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Thank you for your email. I am sorry to hear that you have been experiencing problems with his antibody.

I have checked the details you have kindly provided; however I would like to ask few questions;

- Was the secondary antibody f...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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