Factor VII人Chromogenic Activity Assay试剂盒(ab108830)

概述

  • 产品名称Factor VII人Chromogenic Activity Assay试剂盒
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型Quantitative
  • 灵敏度
    < 0.001 U/ml
  • 检测时间
    3h 00m
  • 种属反应性
    与反应: Human
  • 产品概述

    Factor VII Human Chromogenic Activity Assay Kit (ab108830) has been developed to determine human FVII activity in plasma, serum and cell culture supernatants. The assay couples immunofunctional and indirect amidolytic assay. A polyclonal antibody specific for Human FVII has been pre-coated onto a microplate and active FVII is bound to the immobilized antibody. The assay measures the ability of lipoprotein TF/FVIIa to activate factor X (FX) to factor Xa. The amidolytic activity of the TF/FVIIa complex is quantitated by the amount of FXa produced using a highly specific FXa substrate releasing a yellow para-nitroaniline (pNA) chromophore. The change in absorbance of the pNA at 405 nm is directly proportional to the FVII enzymatic activity.

  • 说明

    Factor VII (FVII) is a vitamin K-dependent plasma glycoprotein that is synthesized in the liver and circulates in blood as a single-chain inactive zymogen with a molecular mass of 50 kDa. Upon tissue damage and vascular injury, the cell surface receptor and cofactor tissue factor (TF) binds and allosterically activates FVII to its active form, FVIIa. The TF/FVIIa complex catalyzes the conversion of both factor IX to factor IXa and factor X to factor Xa to initiate coagulation via the extrinsic pathway. Very low levels of FVII are associated with severe coagulation disorders. Elevated plasma levels of FVII coagulant activity constitute an independent risk factor for fatal outcomes of coronary heart disease in middle-aged men.

  • 经测试应用适用于: Functional Studiesmore details

性能

    应用

    Our Abpromise guarantee covers the use of ab108830 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    应用 Ab评论 说明
    Functional Studies Use at an assay dependent dilution.

    Factor VII Human Chromogenic Activity Assay Kit 图像

    • Factor VII measured in biological fluids showing quantity (IU) per mL of tested sample

    • Standard Curve

    实验方案

    Factor VII Human Chromogenic Activity Assay Kit (ab108830)参考文献

    ab108830 has not yet been referenced specifically in any publications.

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    The Factor VII Human Chromogenic Activity Assay Kit had been developed to measure human FVII activity in plasma and cell cul...

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    Thank you for your enquiry.  I can confirm that this kit can measure both Factor VII and Factor VIIa activity since this kit will activite Factor VII to become Factor VIIa by tissue factor. However, this kit will not be able to differentiate between...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"