Anti-Factor V Heavy Chain抗体[AHV-5146] (ab61348)

概述

  • 产品名称
    Anti-Factor V Heavy Chain抗体[AHV-5146]
    参阅全部 Factor V Heavy Chain 一抗
  • 描述
    小鼠单克隆抗体[AHV-5146] to Factor V Heavy Chain
  • 特异性
    This antibody is specific for Human factor V and Va, heavy chain (fragment D, 94,000) of factor Va.
  • 经测试应用
    适用于: ICC/IF, WB, ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length native protein (purified) corresponding to Human Factor V Heavy Chain.

  • 表位
    Between Arg 306 - Arg 506.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C.
  • 存储溶液
    Preservative: None
    Constituents: 50% (v/v)Glycerol/water
  • Concentration information loading...
  • 纯度
    >95% by SDS-PAGE
  • 克隆
    单克隆
  • 克隆编号
    AHV-5146
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab61348 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/2000.
WB Use at an assay dependent concentration. Predicted molecular weight: 94 kDa.
ELISA Use at an assay dependent concentration.

靶标

  • 相关性
    Factor V is an essential factor of the blood coagulation cascade. This factor circulates in plasma, and is converted to the active form by the release of the activation peptide by thrombin during coagulation. This generates a heavy chain and a light chain which are held together by calcium ions.
  • 细胞定位
    Secreted
  • 数据库链接
  • 别名
    • Activated protein C cofactor antibody
    • Coagulation factor V antibody
    • Coagulation factor V heavy chain antibody

Anti-Factor V Heavy Chain antibody [AHV-5146] 图像

  • Immunofluorescence analysis of pMT2/FV tranfected COS-1 cells, staining Factor V Heavy Chain with ab61348.

    Cells were fixed with formaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 1% BSA for 30 minutes at 37°C. Samples were incubated with primary antibody (1/2000 in 0.1% Triton X-100 in PBS) for 1 hour at 37°C. An FITC-conjugated goat anti-mouse polyclonal IgG (1/100) was used as the secondary antibody.

    See Abreview

Anti-Factor V Heavy Chain antibody [AHV-5146] (ab61348)参考文献

ab61348 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (pMT2/FV tranfected COS-1 cell)
Specification
pMT2/FV tranfected COS-1 cell
Fixative
Formaldehyde
Permeabilization
Yes - 0.1% Triton X-100 in PBS
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 37°C
Username

Abcam user community

Verified customer

提交于 Nov 21 2012

Thank you for contacting us.

The clone number for ab61348 is AHV-5146 and for ab61413 it is AHV-5108.

I will send you the clone nunmber for ab1015 soon.

I hope this information is helpful to you. Please do not hesitate to con...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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