Anti-Factor V Heavy Chain抗体(ab61348)

概述

  • 产品名称Anti-Factor V Heavy Chain抗体
    参阅全部 Factor V Heavy Chain 一抗
  • 描述
    小鼠单克隆抗体to Factor V Heavy Chain
  • 特异性This antibody is specific for Human factor V and Va, heavy chain (fragment D, 94,000) of factor Va.
  • 经测试应用适用于: ICC/IF, WB, ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length native purified Human Factor V Heavy Chain

  • 表位Between Arg 306 - Arg 506.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 50% (v/v)Glycerol/water
  • Concentration information loading...
  • 纯度Ion Exchange Chromatography
  • 纯化说明Salt fractionation followed by gel filtration and ion exchange chromatography.
  • 克隆单克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab61348 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/2000.
WB Use at an assay dependent dilution. Predicted molecular weight: 94 kDa.
ELISA Use at an assay dependent dilution.

靶标

  • 相关性Factor V is an essential factor of the blood coagulation cascade. This factor circulates in plasma, and is converted to the active form by the release of the activation peptide by thrombin during coagulation. This generates a heavy chain and a light chain which are held together by calcium ions.
  • 细胞定位Secreted
  • 数据库链接
  • 别名
    • Activated protein C cofactor antibody
    • Coagulation factor V antibody
    • Coagulation factor V heavy chain antibody

Anti-Factor V Heavy Chain antibody 图像

  • Immunofluorescence analysis of pMT2/FV tranfected COS-1 cells, staining Factor V Heavy Chain with ab61348.

    Cells were fixed with formaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 1% BSA for 30 minutes at 37°C. Samples were incubated with primary antibody (1/2000 in 0.1% Triton X-100 in PBS) for 1 hour at 37°C. An FITC-conjugated goat anti-mouse polyclonal IgG (1/100) was used as the secondary antibody.

    See Abreview

Anti-Factor V Heavy Chain antibody (ab61348)参考文献

ab61348 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (pMT2/FV tranfected COS-1 cell)
Specification pMT2/FV tranfected COS-1 cell
Fixative Formaldehyde
Permeabilization Yes - 0.1% Triton X-100 in PBS
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 37°C
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Verified customer

提交于 Nov 21 2012

Thank you for contacting us.

The clone number for ab61348 is AHV-5146 and for ab61413 it is AHV-5108.

I will send you the clone nunmber for ab1015 soon.

I hope this information is helpful to you. Please do not hesitate to con...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"